CSF1R 蛋白, Cynomolgus (HEK293, His)

CSF1R is colony-stimulating factor receptor, a type I transmembrane protein receptor. Binding of CSF1R to CSF-1 or IL-34 activates a cascade of extracellular pro-cell survival kinases, including PI3K, ERK1/2, and JNK. CSF1R also recruits multiple downstream effector proteins/pathways, including PI3K, Grb2, SFK, Src, PKC, etc. CSF1R is involved in embryonic development and tissue repair and is associated with tumorigenesis and cancer progression. CSF1R Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CSF1R protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CSF1R Protein, Cynomolgus (HEK293, His) is 498 a.a., with molecular weight of 80-120 kDa.

集落刺激因子 1 受体 (CSF1R) 是一种 I 型跨膜蛋白受体和酪氨酸激酶，与细胞存活、增殖和分化有关。集落刺激因子-1 (CSF-1) 和白介素-34 (IL-34) 是 CSF1R 的细胞外配体，这种内源性细胞因子与 CSF1R 的胞外域连接激活，会触发细胞内酪氨酸激酶结构域的自磷酸化，随后激活多个下游促生存激酶级联，包括 PI3K、ERK1/2 和 JNK。CSF1R 的胞内结构域含有自磷酸化酪氨酸残基，然后招募多种下游效应蛋白/通路并与其复合，包括 PI3K、Grb2、SFK、Src、PKC 等。CSF1R 普遍表达于巨噬细胞、小胶质细胞、破骨细胞和骨髓树突状细胞中，但在造血干细胞中很少表达。研究发现，CSF1R 参与胚胎发育和组织修复，与肿瘤发生和癌症进展有关。CSF1R 能够调节肿瘤相关巨噬细胞 (TAM)，使 TAM 释放致瘤细胞因子，进一步使肿瘤微环境失调，形成促肿瘤表型。CSF1R 突变还与神经退行性疾病、骨骼异常有关。

Measured by its ability to inhibit the M-CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.003272 µg/mL in the presence of 1 ng/mL of Recombinant Human M-CSF, corresponding to a specific activity is 3.06×10⁵ units/mg.

• 
  Measured by its ability to inhibit the M-CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.003272 µg/mL in the presence of 1 ng/mL of Recombinant Human M-CSF，corresponding to a specific activity is 3.06×10⁵ units/mg.

Cynomolgus

HEK293

C-6*His

A0A2K5WG90/XP_005558297.1 (I20-P517)

102124214  [NCBI]

IPVIEPSGPELVVKPGETVTLRCVGNGSVEWDGPISPHWTLYSDGPSSVLTTNNATFQNTRTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAKEVVVFEDQDALLPCLLTDPVLEAGVSLVRLRGRPLLRHTNYSFSPWHGFIIHRAKFIQGQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASNIDVDFDVFLQHNTTKLAIPQRSDFHDNRYQKVLTLSLGQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLDLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANATTKDTYRHTFTLSLPRLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTSINGSGTLLCAASGYPQPNVTWLQCAGHTDRCDEAQVLQVWVDPHPEVLSQEPFQKVTVQSLLTAETLEHNQTYECRAHNSVGSGSWAFIPISAGARTHPPDEFLFTP

80-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.