MAD2L1 蛋白, Human (His-SUMO)

MAD2L1 is a critical spindle assembly checkpoint component that delays anaphase until correct chromosome alignment. During prophase, it forms a heterotetrameric complex with MAD1L1 at unattached kinetochores, recruiting O-MAD2 and aiding transformation. MAD2L1 Protein, Human (His-SUMO) is the recombinant human-derived MAD2L1 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

MAD2L1 是纺锤体组装检查点的重要组成部分，在防止后期开始直至中期板实现正确的染色体排列方面发挥着关键作用。在前期，处于闭合构象的 MAD2L1 与 MAD1L1 在未附着的动粒处形成异四聚体复合物，募集 MAD2L1 (O-MAD2) 的开放构象分子并促进向闭合构象的转化。MAD2L1 对执行有丝分裂检查点至关重要，它监测着丝粒-纺锤体附着，通过隔离 CDC20 抑制后期促进复合物，并确保后期启动前染色体的排列。MAD2L1 可以作为单体、同二聚体或与 MAD1L1 的异二聚体存在，形成四聚体核心复合物。它与多种蛋白质相互作用，包括 MAD2L1BP、ADAM17/TACE、CDC20、BUB1B、TTK、TPR、UBD、NEK2 和 HSF1，在细胞周期调节和有丝分裂进展中发挥多方面作用。与 MAD1L1 亚型的相互作用导致细胞质隔离，为 MAD2L1 的调节功能增加了一层额外的复杂性。

Human

E. coli

N-SUMO;N-6*His

Q13257-1 (A2-D205)

4085  [NCBI]

ALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND

Approximately 39.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HC1,1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.