MAdCAM1 蛋白, Human (HEK293, His)

MAdCAM1 is a cell adhesion leukocyte receptor expressed primarily by mucosal venules and plays a critical role in coordinating lymphocyte trafficking into mucosal tissues, particularly Peyer's patches and intestinal lamina propria. This protein exhibits the ability to bind integrins α-4/β-7 and L-selectin, thereby regulating the passage and retention of leukocytes in the mucosal environment. MAdCAM1 Protein, Human (HEK293, His) is the recombinant human-derived MAdCAM1 protein, expressed by HEK293 , with C-6*His labeled tag.

粘膜寻址细胞粘附分子 1 (MAdCAM1) 是一种在粘膜小静脉内皮细胞上表达的细胞粘附白细胞受体，在引导淋巴细胞流入粘膜组织（特别是派尔氏淋巴结和肠固有层）方面发挥着至关重要的作用。MAdCAM1 作为整合素 α-4/β-7 和 L-选择素的结合伴侣，从而调节粘膜组织中白细胞的通过和保留。MAdCAM1 的同二聚体结构有助于其介导内皮细胞和循环免疫细胞之间粘附的功能。值得注意的是，缺乏粘蛋白样结构域的亚型 2 可能具有支持整合素 α-4/β-7 依赖性粘附强化的特殊功能，而不依赖于 L-选择素结合。了解 MAdCAM1 的特征和相互作用可以深入了解粘膜组织中控制淋巴细胞运输的复杂机制。参考资料：所提供的信息基于截至 2022 年 1 月的一般知识，未引用具体参考资料。

1.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells in the presence of 1 mM MnCl2. The ED₅₀ for this effect is 1.987 μg/mL, corresponding to a specific activity is 503.2 units/mg.
2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells. When 5×10⁴ cells/well are added to Human MAdCAM-1 coated plates (2.5 μg/mL with 100 µL/well), approximately 52.575% will adhere after 1 hour incubation at 37 °C in the presence of 1 mM MnCl2.

• 
  Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells in the presence of 1 mM MnCl2. The ED₅₀ for this effect is 1.987 μg/mL, corresponding to a specific activity is 503.2 units/mg.

Human

HEK293

C-6*His

Q4PKD0 (Q19-Q333)

/

QSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSQEPPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGHTQGKVIPTGSSKPAGDQ

Approximately 55-75 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.