MAG/Siglec-4a 蛋白, Mouse (HEK293, His, solution)

The MAG/Siglec-4a protein mediates myelinating cell-neuron interactions by binding to gangliosides, RTN4R, and RTN4RL2. It maintains normal axon myelination and protects motoneurons from apoptosis after injury. It prevents degeneration of myelinated axons in adults by binding to gangliosides. Moreover, it inhibits neurite outgrowth by interacting with RTN4R, RTN4RL2, and gangliosides. It can exist as a monomer or homodimer and interacts with BSG isoform 2. MAG/Siglec-4a Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived MAG/Siglec-4a protein, expressed by HEK293, with C-6*His labeled tag. The total length of MAG/Siglec-4a Protein, Mouse (HEK293, His, solution) is 497 a.a., with molecular weight of 70-120 kDa.

MAG/Siglec-4a 蛋白是一种粘附分子，在介导髓鞘细胞和神经元之间的相互作用中发挥着至关重要的作用。它通过与含有唾液酸的神经节苷脂以及糖蛋白 RTN4R 和 RTN4RL2 结合来实现这一点。虽然它对于初始髓鞘形成不是必需的，但它参与维持正常的轴突髓鞘形成。此外，它可能通过与神经元 RTN4R 和 RTN4RL2 的相互作用，提供针对运动神经元凋亡的保护，特别是在损伤后。在成人中，它对于防止有髓轴突变性至关重要，并且该过程可能依赖于它与轴突细胞膜上的神经节苷脂的结合。此外，MAG/Siglec-4a 蛋白通过抑制轴突纵向生长，充当神经突生长的负调节因子。其对神经突延伸的抑制作用主要通过其与神经元 RTN4R、RTN4RL2 和神经节苷脂的相互作用来介导。它优先与 α-2,3-连接的唾液酸和神经节苷脂 Gt1b 结合。MAG/Siglec-4a 蛋白可以单体或同二聚体的形式存在，并通过其前三个 N 端 Ig 样结构域与 BSG 的亚型 2 相互作用。

Mouse

HEK293

C-6*His

P20917 (G20-P516)

17136  [NCBI]

GHWGAWMPSTISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAKSLYLDLEEVTPGEDGVYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTIRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP

70-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.