MAG/Siglec-4a 蛋白, Rat (HEK293, His)

MAG/Siglec-4a protein binds gangliosides, RTN4R, and RTN4RL2, mediating myelinating cell-neuron interactions. It maintains axon myelination, protects motoneurons, prevents axon degeneration, and inhibits neurite outgrowth. It exists as a monomer or homodimer, interacting with BSG isoform 2. MAG/Siglec-4a Protein, Rat (HEK293, His) is the recombinant rat-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-10*His labeled tag. The total length of MAG/Siglec-4a Protein, Rat (HEK293, His) is 497 a.a., .

MAG/Siglec-4a 蛋白是一种粘附分子，在介导髓鞘细胞和神经元之间的相互作用中发挥着至关重要的作用。它通过结合神经元唾液酸神经节苷脂以及糖蛋白 RTN4R 和 RTN4RL2 来实现这一点。虽然它对于初始髓鞘形成不是必需的，但它似乎参与维持正常的轴突髓鞘形成并保护运动神经元免于凋亡，即使在损伤后也是如此。该蛋白质还需要防止成人有髓轴突变性，可能是通过其与轴突细胞膜上的神经节苷脂的结合。此外，它还充当神经突生长的负调节剂，通过与 RTN4R 结合抑制轴突纵向生长和轴突生长。其对神经突延伸的抑制作用主要是通过其与神经元 RTN4R、RTN4RL2 和神经节苷脂的相互作用介导的。MAG/Siglec-4a 蛋白可以单体或同二聚体的形式存在，并通过其前三个 N 端 Ig 样结构域与 BSG 的亚型 2 相互作用。

Measured by its ability to inhibit proliferation of SH-SY5Y cells. The ED₅₀ for this effect is 2.501 μg/mL.

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  Measured by its ability to inhibit proliferation of SH-SY5Y cells. The ED₅₀ for this effect is 2.501 μg/mL, corresponding to a specific activity is 399.840 units/mg.

Rat

HEK293

C-10*His

P07722 (G20-P516)

29409

GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIINTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAESLYLDLEEVTPAEDGIYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTLRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP

Approximately 75-95 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.