1. 重组蛋白
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. maiA 蛋白, Serratia marcescens

maiA 蛋白, Serratia marcescens

目录号: HY-P702172
产品使用指南

maiA蛋白是参与催化马来酸C2-C3双键顺反异构化从而形成富马酸的关键酶。通过促进这种化学转化,maiA 在调节马来酸中 C2-C3 双键的异构构型,最终将其转化为富马酸方面发挥着至关重要的作用。maiA 蛋白, Serratia marcescens 是重组的 maiA 蛋白,由 E. coli 表达,不带标签。maiA 蛋白, Serratia marcescens 全长 250 个氨基酸。

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描述

The maiA protein is a key enzyme involved in catalyzing the cis-trans isomerization of the C2-C3 double bond in maleic acid, leading to the formation of fumaric acid. By facilitating this chemical transformation, maiA plays a crucial role in regulating the isomeric configuration of the C2-C3 double bond in maleic acid, ultimately converting it into fumaric acid. maiA Protein, Serratia marcescens is the recombinant maiA protein, expressed by E. coli , with tag free. The total length of maiA Protein, Serratia marcescens is 250 a.a., .

研究背景

maiA 蛋白是一种关键酶,参与催化马来酸中 C2-C3 双键的顺反异构化,从而导致富马酸的形成。通过促进这种化学转化,maiA 在调节马来酸中 C2-C3 双键的异构构型,最终将其转化为富马酸方面发挥着至关重要的作用。这种酶活性在马来酸需要代谢或重定向的代谢途径中非常重要,了解 maiA 的功能可以为这些过程的调节提供见解。

种属

Others

表达系统

E. coli

标签

Tag Free

蛋白编号

Q9KWI0 (M1-Y250)

基因 ID

/

蛋白结构
N-term
maiA (M1-Y250)
Accession # Q9KWI0
C-term
同用名
maiA; Maleate isomerase; Maleate cis-trans isomerase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

maiA Protein, Serratia marcescens 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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