Major capsid 蛋白 VP1 蛋白, Murine polyomavirus (Sf9, His)

The major capsid protein, the VP1 protein, is a 40 nm diameter icosahedral capsid composed of 72 pentamers linked by disulfide bonds. During infection, VP1 participates in nucleosome rearrangement and encapsulates replicated genomic DNA. Major capsid protein VP1 Protein, Murine polyomavirus (Sf9, His) is the recombinant Virus-derived Major capsid protein VP1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Major capsid protein VP1 Protein, Murine polyomavirus (Sf9, His) is 372 a.a., with molecular weight of ~42.5 kDa.

主要衣壳蛋白VP1形成直径40 nm、T=7对称性的二十面体衣壳，包含72个通过二硫键连接的五聚体，并与VP2或VP3蛋白结合。VP1 与细胞表面的末端 α(2,3) 连接的唾液酸相互作用，促进病毒颗粒附着到靶细胞并诱导小窝蛋白介导的内吞作用。在内化过程中，病毒颗粒运输至内质网，VP1 在此经历五聚体间二硫键的异构化，引发初始脱壳。随后，内质网相关的降解机制可能有助于在进入核之前易位至细胞质。核进入涉及VP2/VP3核定位信号的选择性暴露和导入识别。在感染后期，新合成的 VP1 将复制的基因组 DNA 封装在细胞核中，并有助于在病毒 DNA 周围重新排列核小体。VP1通过二硫键连接形成同多聚体，构成由72个二十面体单元组成的病毒衣壳，每个二十面体单元包含5个二硫键连接的VP1拷贝。VP1 还与次要衣壳蛋白 VP2 和 VP3 相互作用。

Virus

Sf9 insect cells

C-His

P24595 (A2-L373)

29031028  [NCBI]

APTVKKRTSQNQGLSPQKSQNSVVVGGIQVLDVRTGPDSITQIEAFLNPRMGKPVDSDFYGFSDNITVSADYTQDMPRIKELPCYSMAKISLPMLNEDMTCDTILMWEAISCKTEVVGVSSLTNCHSAVKRLYDNEGAGFPVQGLNFHFFSVGGEALDLQWLWKNYRCNYPAGVAALQAAPKAAQVLDPKLKAKLTADGKFPIEAWSPDPAKNENTRYFGTYTGGLQTPPVLQITNTTTTILLNENGVGPLCKGDGLYLASADIVGFRTQQNNKMHLRGLPRYFSIHLRKGCANPYPVSSLLNTFSSEMMPLNSWMLQVEEVRIYDGVERLPGDPDMIRYRIIWPGRLLSLIFPAMRHKHLYFFVMQAFIVL

Approximately 42.5 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<40 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.