MAN1B1 蛋白, Human (HEK293, His)

MAN1B1, integral to glycoprotein quality control, trims a single alpha-1,2-linked mannose from Man(9)GlcNAc(2), producing Man(8)GlcNAc(2). In the ERQC, elevated enzyme concentrations allow further trimming to Man(5-6)GlcNAc(2). This enzymatic function is crucial for glycoprotein processing and quality control in the endoplasmic reticulum, ensuring proper folding and maturation. MAN1B1 Protein, Human (HEK293, His) is the recombinant human-derived MAN1B1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MAN1B1 Protein, Human (HEK293, His) is 594 a.a., with molecular weight of 58-80 kDa.

MAN1B1 蛋白在糖蛋白质量控制中发挥着关键作用，参与靶向错误折叠糖蛋白的降解。其主要酶活性涉及从 Man(9)GlcNAc(2) 中修剪单个 α-1,2 连接的甘露糖残基，从而产生 Man(8)GlcNAc(2)。值得注意的是，在酶浓度升高的 ER 质量控制室 (ERQC) 中，MAN1B1 可以进一步将碳水化合物修剪为 Man(5-6)GlcNAc(2)。这种酶的功能凸显了它在内质网内糖蛋白的加工和质量控制中的重要作用，确保这些蛋白质的正确折叠和成熟。

Human

HEK293

C-6*His

Q9UKM7 (D106-A699)

11253  [NCBI]

DHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPIWTPA

58-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 or 20 mM PB, 50 mM NaCl, 5% Sucrose, 5% Mannitol, 0.05% Tween 80, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.