MAPKSP1 蛋白, Human (His)

The MAPKSP1 protein, also known as porphobilinogen deaminase (HMBS), is crucial in the heme biosynthetic pathway and catalyzes the sequential polymerization of four porphobilinogen molecules to form hydroxymethyl bixane. The process begins with the assembly of the dipyrromethane cofactor of porphobilinogen or preuroporphyrinogen. MAPKSP1 Protein, Human (His) is the recombinant human-derived MAPKSP1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of MAPKSP1 Protein, Human (His) is 124 a.a., with molecular weight of ~15.0 kDa.

HMBS，也称为胆色素原脱氨酶，通过催化四分子胆色素原的顺序聚合，导致形成羟甲基联烷，也称为前尿卟啉原，在血红素生物合成途径中发挥着至关重要的作用。催化过程从二吡咯甲烷辅助因子的组装开始，二吡咯甲烷辅助因子由脱辅酶的两个胆色素原分子或前尿卟啉原形成。共价连接的辅因子充当引物，协调其周围的四吡咯产物的组装。在催化的最后一步，产物前尿卟啉原被释放，而辅因子仍然与全脱氨酶完整结合，突出了 HMBS 在血红素合成中复杂而重要的作用。

Human

E. coli

N-6*His

Q9UHA4 (M1-S124)

8649  [NCBI]

MADDLKRFLYKKLPSVEGLHAIVVSDRDGVPVIKVANDNAPEHALRPGFLSTFALATDQGSKLGLSKNKSIICYYNTYQVVQFNRLPLVVSFIASSSANTGLIVSLEKELAPLFEELRQVVEVS

Approximately 15.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 5 mM DTT, 30% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.