1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. MARK1 蛋白, Human (His)

MARK1 蛋白, Human (His)

目录号: HY-P701807
产品使用指南

MARK1 是一种重要的丝氨酸/苏氨酸蛋白激酶,可严格调节细胞极性和微管动力学。它磷酸化微管相关蛋白(DCX、MAP2、MAP4、MAPT/TAU),导致其解离和分解。MARK1 蛋白, Human (His) 是重组的 MARK1 蛋白,由 E. coli 表达,带有 N-6*His 标签。MARK1 蛋白, Human (His) 全长 751 个氨基酸。

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描述

MARK1 is an important serine/threonine protein kinase that critically regulates cell polarity and microtubule dynamics. It phosphorylates microtubule-associated proteins (DCX, MAP2, MAP4, MAPT/TAU), causing their dissociation and disassembly. MARK1 Protein, Human (His) is the recombinant human-derived MARK1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of MARK1 Protein, Human (His) is 751 a.a., .

研究背景

MARK1 是一种丝氨酸/苏氨酸蛋白激酶,在细胞过程中发挥着至关重要的作用,特别是在细胞极性和微管动力学的调节中。它通过磷酸化微管相关蛋白(例如 DCX、MAP2、MAP4 和 MAPT/TAU)来实现这一目标,导致它们与微管分离并随后分解。在神经元迁移的背景下,MARK1 可能通过 DCX 的磷酸化和调节来调节细胞极性和微管动力学的双重活性,有助于其参与这一过程。此外,MARK1 作为 Wnt 信号通路的正调节因子,可能是通过介导散乱蛋白(DVL1、DVL2 和/或 DVL3)的磷酸化,为其在细胞调节中的多方面作用增添了另一层。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9P0L2 (N45-L795)

基因 ID

4139

蛋白结构
N-term
6*His
MARK1 (N45-L795)
Accession # Q9P0L2
C-term
同用名
MARK1; Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MARK1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MARK1 Protein, Human (His)
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HY-P701807
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