1. 重组蛋白
  2. Complement System
  3. MASP-2
  4. MASP2 蛋白, Rat (His)

MASP2 蛋白, Rat (His)

目录号: HY-P77801
产品使用指南

甘露聚糖结合凝集素丝氨酸蛋白酶 2 (MASP2) 是一种存在签名注释传播所需的保守残基缺陷的蛋白质。MASP2 中缺失的特定残基阻止了与该蛋白质相关的某些功能特征的传播。MASP2 蛋白, Rat (His) 是重组的 MASP2 蛋白,由 E. coli 表达,带有 C-His 标签。MASP2 蛋白, Rat (His) 全长 399 个氨基酸,分子量为 15-20 kDa。

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描述

Mannan-binding lectin serine protease 2 (MASP2) is a protein defective in conserved residues required for propagation of signature annotations. Specific residues missing in MASP2 prevent the propagation of certain functional features associated with this protein. MASP2 Protein, Rat (His) is the recombinant rat-derived MASP2 protein, expressed by E. coli , with C-His labeled tag. The total length of MASP2 Protein, Rat (His) is 399 a.a., with molecular weight of 15-20 kDa.

研究背景

甘露聚糖结合凝集素丝氨酸蛋白酶 2 (MASP2) 是一种蛋白质,其传播特征注释所需的保守残基存在缺陷。MASP2 中缺失的特定残基阻碍了与该蛋白质相关的某些功能特征的传播。MASP2 是一种丝氨酸蛋白酶,在补体系统的凝集素途径中发挥着至关重要的作用,补体系统是先天免疫反应的一部分。MASP2 与病原体表面的特定碳水化合物结合可激活凝集素途径,从而激活补体成分和随后的免疫反应。MASP2 中缺陷残基对其功能和凝集素途径的影响需要进一步探索,以更好地了解其对 MASP2 介导的免疫反应的影响。

种属

Rat

表达系统

E. coli

标签

C-His

蛋白编号

A2VCV7 (T287-F685)

基因 ID
蛋白结构
N-term
MASP2 (T287-F685)
Accession # A2VCV7
His
C-term
同用名
MAP19; MASP-2; MASP1P1; sMap
分子量

15-20 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MASP2 Protein, Rat (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MASP2 Protein, Rat (His)
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