MD2 蛋白, Human (His)

MD2 protein binds bacterial lipopolysaccharide (LPS) and collaborates with TLR4 in the innate immune response to LPS. It also interacts with TLR2 in the response to cell wall components from bacteria. MD2 enhances TLR4-dependent NF-kappa-B activation and forms a heterogeneous homomer, participating in a multi-protein complex with CD14 and TLR4. Ligand binding induces complex oligomerization, crucial for the cellular response to LPS. MD2 Protein, Human (His) is the recombinant human-derived MD2 protein, expressed by E. coli , with C-His labeled tag. The total length of MD2 Protein, Human (His) is 142 a.a., with molecular weight of 18.07 kDa.

MD2 蛋白是先天免疫反应的关键参与者，是识别细菌脂多糖 (LPS) 和协调免疫反应的关键成分。它直接与 LPS 结合，并与 TLR4 协作，引发针对细菌 LPS 的先天免疫反应。此外，MD2 与 TLR2 协同作用，对革兰氏阳性和革兰氏阴性细菌的细胞壁成分做出反应。MD2 与 TLR4 的相互作用增强了 NF-kappa-B 的激活，强调了其在信号通路中的作用。在同时表达 LY96 和 TLR4 的细胞环境中，MD2 能够对 LPS 做出反应，强调了它在细胞识别细菌成分中的重要性。在结构上，MD2 由通过二硫键连接的同二聚体形成异质同聚体，是脂多糖 (LPS) 受体复合物的重要组成部分，该复合物至少包括 CD14、LY96 和 TLR4。MD2 的配体结合诱导与 TLR4 的相互作用，并促进复合物的寡聚化，进一步凸显其在针对细菌挑战的免疫反应中的核心作用。

Human

E. coli

C-His

Q9Y6Y9-1 (Q19-N160)

/

QKQYWVCNSSDASISYTYCDKMQYPISINVNPCIELKRSKGLLHIFYIPRRDLKQLYFNLYITVNTMNLPKRKEVICRGSDDDYSFCRALKGETVNTTISFSFKGIKFSKGKYKCVVEAISGSPEEMLFCLEFVILHQPNSN

18.07 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in 4 mM HCL. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 4 mM HCl.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.