MeCP2 蛋白, Human (HEK293, His)

The MeCP2 protein is a chromosome binder that specifically binds to methylated DNA independent of flanking sequences. MeCP2 Protein, Human (HEK293, His) is the recombinant human-derived MeCP2 protein, expressed by HEK293 , with C-6*His labeled tag.

MeCP2 蛋白作为染色体实体发挥作用，具有特异性结合甲基化 DNA 的独特能力，独立于侧翼 DNA 序列靶向单个甲基 CpG 对。MeCP2 作为转录抑制的介质，与组蛋白脱乙酰酶和辅阻遏物 SIN3A 相互作用。此外，它表现出双重结合能力，与含有 5-甲基胞嘧啶 (5mC) 和 5-羟甲基胞嘧啶 (5hmC) 的 DNA 结合，表现出对 5-甲基胞嘧啶 (5mC) 的显着偏好。MeCP2 与 FNBP3、CDKL5、ATRX、NCOR2、TBL1XR1 和 TBL1X 的相互作用进一步强调了 MeCP2 的多功能性，每种相互作用都有助于各种细胞过程，包括共阻遏物复合物的招募和异染色质组织的协调。这种多方面的功能强调了 MeCP2 在表观遗传调控和基因表达调节中的关键作用。

Human

HEK293

C-6*His

P51608-1 (M1-S486)

4204  [NCBI]

MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS

Approximately 83.0-90.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 5% Trehalose, 2 mM DTT, pH 8.0 or 20 mM Histidine-HCl, 8% Sucrose, 50mM NaCl, 0.02% Tween 80, pH 6.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.