1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. MEK1 蛋白, Mouse (sf9, His-GST)

MEK1 蛋白, Mouse (sf9, His-GST)

目录号: HY-P73825
产品使用指南

MEK1 蛋白是一种双特异性激酶,在 MAP 激酶通路中发挥着至关重要的作用。MEK1 调节各种细胞过程,如生长、粘附、存活和分化,以及转录、代谢和细胞骨架重排。MEK1 蛋白, Mouse (sf9, His-GST) 是重组的 MEK1 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。

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描述

The MEK1 protein is a dual-specificity kinase that plays a crucial role in the MAP kinase pathway.MEK1 regulates various cellular processes such as growth, adhesion, survival, and differentiation, as well as transcription, metabolism, and cytoskeletal rearrangements.MEK1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived MEK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

研究背景

MEK1 蛋白是 MAP 激酶信号转导通路的关键组成部分,在将细胞外信号转变成细胞内反应方面发挥着至关重要的作用。当生长因子和细胞因子等配体与其细胞表面受体结合时,MEK1 作为 RAS-RAF1-MEK1/2-MAPK/ERK 级联的一部分被激活。MEK1 与 MAP2K2/MEK2 一起催化 ERK1ERK2 激活环中苏氨酸和酪氨酸残基的双重磷酸化,从而启动下游信号传导事件。该级联调节多种生物功能,包括细胞生长、粘附、存活和分化。除了在转录和细胞骨架调节中发挥作用外,MAPK/ERK 通路还会影响过氧化物酶体增殖物激活受体 γ (PPARG),从而促进分化和凋亡等细胞过程。此外,MEK1 参与有丝分裂期间的内体动力学和高尔基体碎裂,强调了其在协调复杂的细胞反应方面的多功能性。

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

P31938 (M1-I393)

基因 ID
蛋白结构
N-term
His-GST
MEK1 (M1-I393)
Accession # P31938
C-term
同用名
Dual specificity mitogen-activated protein kinase kinase 1; MAPKK 1; MEK 1; Map2k1
分子量

Approximately 65 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MEK1 Protein, Mouse (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MEK1 Protein, Mouse (sf9, His-GST)
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HY-P73825
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