Mesothelin 蛋白, Human (Biotinylated, HEK293, His-Avi)

Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein, as cells overexpressing mesothelin have altered adhesive properties. Mesothelin is also a Megakaryocyte-potentiating factor (MPF) which potentiates megakaryocyte colony formation in vitro. Mesothelin may be related to cancer metastasis. Mesothelin Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Mesothelin protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag. The total length of Mesothelin Protein, Human (Biotinylated, HEK293, His-Avi) is 303 a.a., with molecular weight of 28-37 kDa.

Mesothelin 是一种糖基化磷脂酰肌醇锚定的细胞表面蛋白，可能作为细胞黏附蛋白发挥作用，因为过度表达 Mesothelin 的细胞改变了黏附特性。Mesothelin 在上皮性间皮瘤、卵巢癌和特定的鳞状细胞癌中过度表达。可变剪切产生多种 Mesothelin 的转录变体。Mesothelin 也是一种巨核细胞增强因子(MPF)，在体外可增强巨核细胞集落形成^[1][2]。

Human

HEK293

C-Avi;C-6*His

AAH09272.1 (E296-S598)

10232  [NCBI]

EVEKTACPSGKKAPEIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALS

28-37 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.