MFN2 蛋白, Human (Cell-Free, His)

MFN2, a mitochondrial outer membrane GTPase, regulates mitochondrial dynamics, balancing fusion and fission for dynamic organelle morphology. Its overexpression forms extensive mitochondrial networks, highlighting its organizational role. Beyond shaping structure, MFN2 influences mitochondrial metabolism, impacting obesity and apoptosis. In vascular smooth muscle cells, it regulates proliferation. Crucial in mitochondrial quality control, MFN2 facilitates mitophagy and modulates the unfolded protein response during ER stress, emphasizing its diverse roles in cellular homeostasis and stress responses. MFN2 Protein, Human (Cell-Free, His) is the recombinant human-derived MFN2 protein, expressed by E. coli Cell-free, with N-10*His labeled tag. The total length of MFN2 Protein, Human (Cell-Free, His) is 757 a.a., with molecular weight of 87.9 kDa.

MFN2 是一种线粒体外膜 GTP 酶，通过介导聚类和融合过程来复杂地控制线粒体动力学。这种 GTP 酶协调线粒体融合和裂变之间的平衡，关键地塑造这些细胞器的动态形态。值得注意的是，它的过度表达诱导了广泛的线粒体网络的形成，强调了它在线粒体组织中的关键作用。MFN2 的 GTP 酶活性对于膜聚集至关重要，并且可能涉及卷曲螺旋结构域中的显着重排。除了其结构贡献之外，MFN2 还成为线粒体代谢的核心参与者，这表明它与肥胖和细胞凋亡过程有关。此外，它还调节血管平滑肌细胞增殖，证明其在细胞功能中的多种作用。在线粒体质量控制领域，MFN2 有助于通过选择性自噬（线粒体自噬）促进 PRKN 募集至功能失调的线粒体，从而有助于清除受损的线粒体。此外，它在内质网 (ER) 应激期间调节未折叠蛋白反应 (UPR) 方面发挥着至关重要的作用，充当 EIF2AK3 的上游调节因子并在基础条件下抑制其激活。这种多方面的功能强调了 MFN2 在细胞稳态和应激反应机制中的重要性。

Human

E. coli Cell-free

N-10*His

O95140 (M1-R757)

9927

MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVEGTDGHEAFLLTEGSEEKRSAKTVNQLAHALHQDKQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKHFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRSQAGDRIFFVSAKEVLNARIQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHMAAREQQVYCEEMREERQDRLKFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDDYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR

87.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.