MGAT5 蛋白, Human (HEK293, His)

The MGAT5 protein regulates the cytoskeleton, adhesion, migration, and signaling. MGAT5 Protein, Human (HEK293, His) is the recombinant human-derived MGAT5 protein, expressed by HEK293 , with C-His labeled tag. The total length of MGAT5 Protein, Human (HEK293, His) is 553 a.a., with molecular weight of 75 kDa.

MGAT5 蛋白是一种关键酶，可催化 N-乙酰葡糖胺 (GlcNAc) 以 β1-6 连接方式添加到双触角 N-连接寡糖的 α-连接甘露糖上。这种催化活性对于多种蛋白质（包括 EGFR、TGFR（TGF-β 受体）和 CDH2）上发现的支链复合型 N-聚糖的生物合成至关重要。通过参与复杂的 N-聚糖生物合成，MGAT5 在细胞信号传导途径、肌动蛋白细胞骨架重组、细胞间粘附和细胞迁移中发挥着关键作用。值得注意的是，MGAT5 依赖性 EGFR N-糖基化通过阻止快速内吞作用来延长 EGFR 信号传导。MGAT5 对于 TGFB1 及其受体之间的有效相互作用也至关重要，可增强由 FGF2、PDGF、IGF、TGFB1 和 EGF 等生长因子激活的细胞内信号传导途径。此外，MGAT5 介导的 CDH2 N-糖基化可抑制同型细胞间粘附，影响下游信号通路并促进细胞迁移。在免疫反应中，MGAT5依赖性TCR N-糖基化限制TCR聚集并抑制TCR介导的对抗原的反应，有助于正常白细胞逃逸和在炎症部位积聚。此外，MGAT5 抑制单核细胞附着于血管内皮和随后的血细胞渗出，同时还通过促进 FGF2 从细胞外基质的释放来促进内皮细胞增殖和血管生成。总之，MGAT5 作为一种多方面的调节剂而出现，对细胞过程、免疫反应和血管生成具有重大影响。

Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is <0.1 μg/mL, corresponding to a activity is >1×10^4 units/mg.

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  Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is 0.01096 μg/mL, corresponding to a specific activity is 9.124×10⁴ units/mg.

Human

HEK293

C-His

Q09328 (L189-L741)

4249  [NCBI]

LSEVENWCPHLPWRAKNPYEEADHNSLAEIRTDFNILYSMMKKHEEFRWMRLRIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGLLTKESGFKIAETAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHTPDNSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHATVYGSSTKNIPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVDLNNQEEVEDAVKAILNQKIEPYMPYEFTCEGMLQRINAFIEKQDFCHGQVMWPPLSALQVKLAEPGQSCKQVCQESQLICEPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNKHCVFQGDLLLFSCAGAHPRHQRVCPCRDFIKGQVALCKDCL

Approximately 72-78 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.