MIF 蛋白, Human (HEK293, hFc)

The MIF protein is a pro-inflammatory cytokine that is critical for the innate immune response against bacterial pathogens. Its presence at sites of inflammation suggests a role in modulating macrophage function to promote host defense. MIF Protein, Human (HEK293, hFc) is the recombinant human-derived MIF protein, expressed by HEK293 , with C-hFc labeled tag. The total length of MIF Protein, Human (HEK293, hFc) is 114 a.a., with molecular weight of 41.3 kDa.

MIF 蛋白是一种促炎细胞因子，在针对细菌病原体的先天免疫反应中发挥着至关重要的作用。它在炎症部位的表达表明它参与调节宿主防御中的巨噬细胞功能。MIF 抵消糖皮质激素的抗炎作用。尽管MIF在体外具有苯丙酮酸互变异构酶和多巴色素互变异构酶活性，但MIF的生理底物仍不清楚。目前尚不清楚互变异构酶活性是否与其细胞因子活性相关。

Measured by its binding ability in a functional ELISA. Immobilized MIF at 2 μg/mL can bind Anti- MIF Rabbit Monoclonal Antibody, the EC₅₀ is ≤69 ng/mL.

Human

HEK293

C-hFc

P14174 (P2-A115)

4282  [NCBI]

PMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA

45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.