MIP-1 alpha/CCL3 蛋白, Mouse (His)

MIP-1 alpha/CCL3 Protein, Mouse (His), an important chemokine, is a key regulator of immune microenvironment and primarily mediates the trafficking of immune cells in both inflammation and cancer. MIP-1 alpha/CCL3 Protein, Mouse (His) is a recombinant mouse CCL3  (A24-A92) expressed by E.coil with a His tag^[1].

CCL3也称为巨噬细胞炎症蛋白1-a，是CC亚家族的成员。众所周知，CCL3是由单核细胞/巨噬细胞、淋巴细胞、中性粒细胞以及嗜碱性粒细胞、肥大细胞、成纤维细胞和树突状细胞等免疫细胞产生的。同时，CCL3通过与其三种细胞表面受体（包括CCR1、CCR3和CCR5）结合发挥多种生物学效应。MIP-1a诱导白细胞趋化等多种促炎活性，并促进T细胞从血液循环进入炎症组织区域。趋化性CD4+细胞、CD8+细胞、自然杀伤细胞、树突状细胞等与相应受体结合，通过血管内皮细胞迁移来协调免疫反应位点免疫反应的发生。此外，MIP-1a 被认为是肉芽肿、哮喘、T1D 以及其他自身免疫性疾病的关键炎症介质^[1]。

Measured by its ability to chemoattract BaF3 mouse proB cells transfected with mouse CCR5. The ED₅₀ for this effect is 2.771 ng/mL, corresponding to a specific activity is 3.609×10⁵ U/mg.

Mouse

E. coli

N-6*His

P10855/Q5SVU3 (A24-A92)

20302  [NCBI]

HHHHHHAPYGADTPTACCFSYSRKIPRQFIVDYFETSSLCSQPGVIFLTKRNRQICADSKETWVQEYITDLELNA

Approximately 15 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM Tris,150 mM NaCl, 5% Trehalose, 1 mM EDTA, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zhang G, et al. CCL3 participates in the development of rheumatoid arthritis by activating AKT. Eur Rev Med Pharmacol Sci. 2018 Oct;22(20):6625-6632.  [Content Brief]

  [2]. Ioannis Ntanasis-Stathopoulos, et al. CCL3 Signaling in the Tumor Microenvironment. Adv Exp Med Biol. 2020;1231:13-21.  [Content Brief]

  [3]. Giovanni Bernardini, et al. CCL3 and CXCL12 regulate trafficking of mouse bone marrow NK cell subsets. Blood. 2008 Apr 1;111(7):3626-34.  [Content Brief]