CCL4 蛋白, Human

CCL4 Protein, Human is a small cytokine of the CC chemokine subfamily that binds to the CCR5 chemokine receptor on the cell surface, promotes leukocyte aggregation under various inflammatory conditions, and contributes to immune protection against human immunodeficiency virus type 1. CCL4 Protein, Human is a recombinant human CCL4 (A24-N92) expressed by E. coli^[1].

CCL4又名巨噬细胞炎症蛋白（MIP-1β），属于CC趋化因子家族，是人体中由CCL4基因编码的蛋白质，属于17号染色体17q11-q21区域，分子量约为8-10 kDa。CCL4 可由单核细胞、B 细胞、T 细胞、NK 细胞、树突状细胞、中性粒细胞、成纤维细胞、内皮细胞和上皮细胞产生^[1]。CCL4 充当趋化因子，与 G 蛋白偶联受体 CCR5 和 CCR8 结合，并充当炎症或受损组织部位的自然杀伤细胞、单核细胞和各种其他免疫细胞的趋化剂。与此同时，CCL4 还可以作为 CD8+ T 细胞产生的主要 HIV 抑制因子，诱导不同毒株的 HIV-1、HIV-2 和猴免疫缺陷病毒 (SIV) 的剂量依赖性抑制。其中，N端加工形式CCL4（3-69）是由外周血淋巴细胞分泌后蛋白水解裂解产生的，保留了诱导趋化因子受体CCR5表面表达下调并抑制CCR5介导的HIV-1进入的能力进入 T 细胞^[2]。

1. The ED₅₀ is <100 ng/mL as measured by CHO-K1/Gα15/hCCR5 cells (human Gα15 and human CCR5 stably expressed in CHO-K1 cells).
2. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ this effect is 2.785 ng/mL, corresponding to a specific activity is 3.59×10⁵ U/mg.

• 
  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 2.785 ng/mL, corresponding to a specific activity is 3.59×10⁵ U/mg.

Human

E. coli

Tag Free

P13236 (A24-N92)

6351  [NCBI]

APMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN

7-17 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. S G Irving, et al. Two inflammatory mediator cytokine genes are closely linked and variably amplified on chromosome 17q. Nucleic Acids Res. 1990 Jun 11;18(11):3261-70.  [Content Brief]

  [2]. Patricia Menten, et al. Macrophage inflammatory protein-1. Cytokine Growth Factor Rev. 2002 Dec;13(6):455-81.  [Content Brief]