MIP-3 beta/CCL19 蛋白, Human

The MIP-3 beta/CCL19 protein exhibits multifaceted effects affecting lymphocyte recycling, homing, and immune responses. It is involved in T cell trafficking in the thymus and guides T cells and B cells to secondary lymphoid organs by binding to CCR7. MIP-3 beta/CCL19 Protein, Human is the recombinant human-derived MIP-3 beta/CCL19 protein, expressed by E. coli , with tag free.

MIP-3 beta/CCL19 蛋白被认为具有多方面的作用，不仅限于炎症和免疫反应，还包括正常的淋巴细胞再循环和归巢。它可能参与胸腺中 T 细胞的运输，以及 T 细胞和 B 细胞向次级淋巴器官的迁移，强调了它在协调对免疫监视和反应至关重要的细胞运动中的重要性。MIP-3 beta/CCL19 通过与趋化因子受体 CCR7 结合发挥作用，表现出专门针对 T 细胞和 B 细胞的有效趋化活性，将粒细胞和单核细胞排除在其募集之外。此外，它与非典型趋化因子受体 ACKR4 结合，促进 β-arrestin (ARRB1/2) 募集到 ACKR4。值得注意的是，MIP-3 beta/CCL19 通过其 Link 结构域与 TNFAIP6 相互作用，为其复杂的分子关联网络添加了另一层。MIP-3 beta/CCL19 的多种功能凸显了其在免疫细胞动力学中的关键作用，并强调需要全面探索其调控机制。

Measured by its ability to chemoattract Jurkat cells. The ED₅₀ for this effect is 27.79 ng/mL, corresponding to a specific activity is 3.598×10⁴ U/mg.

Human

E. coli

Tag Free

Q99731 (G22-S98)

6363

GTNDAEDCCLSVTQKPIPGYIVRNFHYLLIKDGCRVPAVVFTTLRGRQLCAPPDQPWVERIIQRLQRTSAKMKRRSS

Approximately 11 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.