MLKL 蛋白, Human (His-SUMO)

MLKL protein is a pseudokinase that plays a key role in TNF-induced necroptosis. Despite lacking intrinsic kinase activity, it is activated through RIPK3 phosphorylation, leading to homotrimerization, plasma membrane localization, and execution of necroptosis. MLKL Protein, Human (His-SUMO) is the recombinant human-derived MLKL protein, expressed by E. coli , with N-6*His labeled tag. The total length of MLKL Protein, Human (His-SUMO) is 471 a.a., with molecular weight of ~59 kDa.

MLKL 是一种假激酶，在 TNF 诱导的坏死性凋亡中发挥着关键作用，坏死性凋亡是一种以质膜损伤和钙内流为特征的程序性细胞死亡过程。尽管缺乏内在蛋白激酶活性，MLKL 在被 RIPK3 磷酸化后被激活。这种激活导致同源三聚化、质膜定位和坏死性凋亡的执行。此外，为了应对正粘病毒感染，MLKL 可以经历由 ZBP1 激活引发的核坏死性凋亡，导致核膜破坏并将细胞 DNA 释放到胞质溶胶中。MLKL 的坏死性凋亡功能通过其与高度磷酸化的肌醇磷酸盐（例如肌醇六磷酸 (InsP6)）的结合而得到促进。这种结合不仅介导 N 末端自动抑制区域的释放，而且还需要 RIPK3 依赖性磷酸化才能完全激活。Necrosulfonamide 是一种特异性坏死性凋亡抑制剂，以 Cys-86 为靶点，有效抑制 MLKL 功能。

Human

E. coli

N-6*His

Q8NB16 (M1-K471)

197259

Approximately 59 kDa

Greater than 90 % as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.