MMP-2 蛋白, Mouse (HEK293, His)

MMP-2 protein is a ubiquitous metalloprotease that contributes to vasculature remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. In addition to extracellular matrix degradation, it also targets non-matrix proteins and promotes vasoconstriction. MMP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MMP-2 protein, expressed by HEK293 , with N-His labeled tag.

MMP-2 蛋白是一种多功能、普遍存在的金属蛋白酶，参与多种功能，包括脉管系统重塑、血管生成、组织修复、肿瘤侵袭、炎症和动脉粥样硬化斑块破裂。除了降解细胞外基质蛋白外，它还作用于大内皮细胞 1 和 β 型 CGRP 等非基质蛋白，促进血管收缩。此外，它在 Gly-|-Leu 键处裂解 KISS，似乎通过影响 GSK3beta 活性在心肌细胞死亡途径和心肌氧化应激调节中发挥作用。此外，MMP-2 参与纤维血管组织的形成，其 C 端非催化片段 PEX 具有抗血管生成和抗肿瘤特性，同时抑制细胞迁移和对 FGF2 和玻连蛋白的粘附。此外，MMP-2 作为血管表面整合素 α-v/β-3 的配体。

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is 4214 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

N-His

NP_032636.1 (M409-C662)

17390  [NCBI]

MGLEHSQDPGALMAPIYTYTKNFRLSHDDIKGIQELYGPSPDADTDTGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPTGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWVYSASTLERGYPKPLTSLGLPPDVQQVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADSWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC

Approximately 34.54 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.