1. 重组蛋白
  2. Enzymes & Regulators Biotinylated Proteins
  3. Matrix Metalloproteinases
  4. MMP-9
  5. MMP-9 蛋白, Human (Biotinylated, HEK293, His-Avi)

MMP-9 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P78175
COA 产品使用指南

MMP-9 蛋白是一种基质金属蛋白酶,在局部细胞外基质分解、促进白细胞迁移中发挥着至关重要的作用。建议其可能参与骨破骨细胞吸收。MMP-9 裂解 KiSS1 和 NINJ1,生成它们的分泌形式。它降解 IV 型和 V 型胶原蛋白,产生不同的片段和纤连蛋白,而层粘连蛋白和 Pz 肽不受影响。MMP-9 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 MMP-9 蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。MMP-9 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 688 个氨基酸,分子量为 95-105 kDa。

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规格 价格 是否有货 数量
20 μg ¥1850 In-stock
50 μg ¥3500 In-stock
100 μg ¥6300 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

MMP-9 Protein, a matrix metalloproteinase, plays a crucial role in localized extracellular matrix breakdown, facilitating leukocyte migration. Its potential involvement in bone osteoclastic resorption is suggested. MMP-9 cleaves KiSS1 and NINJ1, generating their secreted forms. It degrades type IV and type V collagen, producing distinct fragments, and fibronectin, while laminin and Pz-peptide remain unaffected. MMP-9 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived MMP-9 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of MMP-9 Protein, Human (Biotinylated, HEK293, His-Avi) is 688 a.a., with molecular weight of 95-105 kDa.

研究背景

MMP-9 蛋白是一种基质金属蛋白酶,在细胞外基质的局部分解中发挥着至关重要的作用,并促进白细胞迁移。有人提出MMP-9也可能参与骨破骨细胞吸收。此外,MMP-9 在 Gly-|-Leu 键和 NINJ1 处裂解 KiSS1,生成分泌形式的 ninjurin-1。此外,已知它会裂解 IV 型和 V 型胶原蛋白,从而产生大的 C 端四分之三片段和较短的 N 端四分之一片段。虽然 MMP-9 降解纤连蛋白,但它对层粘连蛋白或 Pz 肽没有影响。

生物活性

1. Immobilized Biotinylated Human MMP-9 His at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody hFc with the EC50 of 28.8 ng/mL determined by ELISA.
2. Immobilized Anti-MMP-9 Antibody, hFc Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 14.3 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

P14780 (A20-D707)

基因 ID
蛋白结构
N-term
MMP-9 (A20-D707)
Accession # P14780
His-Avi
C-term
同用名
MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
氨基酸序列

APRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGVVVPTRFGNADGAACHFPFIFEGRSYSACTTDGRSDGLPWCSTTANYDTDDRFGFCPSERLYTQDGNADGKPCQFPFIFQGQSYSACTTDGRSDGYRWCATTANYDRDKLFGFCPTRADSTVMGGNSAGELCVFPFTFLGKEYSTCTSEGRGDGRLWCATTSNFDSDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMYPMYRFTEGPPLHKDDVNGIRHLYGPRPEPEPRPPTTTTPQPTAPPTVCPTGPPTVHPSERPTAGPTGPPSAGPTGPPTAGPSTATTVPLSPVDDACNVNIFDAIAEIGNQLYLFKDGKYWRFSEGRGSRPQGPFLIADKWPALPRKLDSVFEERLSKKLFFFSGRQVWVYTGASVLGPRRLDKLGLGADVAQVTGALRSGRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGYVTYDILQCPED

分子量

95-105 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5-8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

MMP-9 Protein, Human (Biotinylated, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MMP-9 Protein, Human (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78175
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