1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. MRE11A 蛋白, Human (His, GST)

MRE11A 蛋白, Human (His, GST)

目录号: HY-P702002
产品使用指南

MRE11A蛋白是DNA双链断裂修复机制的重要组成部分,参与同源重组(HR)途径。它与 RAD50 和 NBS1 相互作用形成 MRN 复合物,这对于感知和修复 DNA 损伤至关重要。MRE11A 蛋白, Human (His, GST) 是重组的 MRE11A 蛋白,由 E. coli 表达,带有 N-6*His, N-GST 标签。MRE11A 蛋白, Human (His, GST) 全长 411 个氨基酸。

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描述

The MRE11A protein is an important component of the DNA double-strand break repair mechanism and participates in the homologous recombination (HR) pathway. It interacts with RAD50 and NBS1 to form the MRN complex, which is essential for sensing and repairing DNA damage. MRE11A Protein, Human (His, GST) is the recombinant human-derived MRE11A protein, expressed by E. coli , with N-6*His, N-GST labeled tag. The total length of MRE11A Protein, Human (His, GST) is 411 a.a., .

研究背景

CXADR 是上皮顶端连接复合物的关键成分,作为同质细胞粘附分子,对于维持紧密连接的完整性至关重要。此外,CXADR 通过与 JAML(白细胞质膜上的跨膜蛋白)粘附相互作用,在介导白细胞跨上皮迁移中发挥关键作用。这种相互作用不仅促进白细胞迁移,而且还激活 γ-δ T 细胞,这是一种存在于上皮组织中参与组织稳态和修复的特殊 T 细胞亚群。与上皮 CXADR 结合后,JAML 通过 PI3 激酶和 MAP 激酶启动 γ-δ T 细胞中的下游信号传导,导致 T 细胞增殖以及细胞因子和生长因子的产生。这些因素反过来又刺激上皮组织的修复过程。值得注意的是,CXADR 还充当 C 型腺病毒的受体,突出了其在微生物感染中的作用。

种属

Human

表达系统

E. coli

标签

N-6*His;N-GST

蛋白编号

P49959 (M1-E411)

基因 ID

4361

蛋白结构
N-term
6*His-GST
MRE11A (M1-E411)
Accession # P49959
C-term
同用名
MRE11; Double-strand break repair protein MRE11; Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MRE11A Protein, Human (His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MRE11A Protein, Human (His, GST)
目录号:
HY-P702002
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