MST1/STK4 蛋白, Human (sf9, His)

MST1/STK4 protein is a stress-activated kinase that is critical in the Hippo signaling pathway and can induce apoptosis and regulate hepatocyte proliferation. Cleaved MST1/STK4 enters the nucleus, causing chromatin condensation and DNA fragmentation. MST1/STK4 Protein, Human (sf9, His) is the recombinant human-derived MST1/STK4 protein, expressed by Sf9 insect cells , with N-10*His labeled tag. The total length of MST1/STK4 Protein, Human (sf9, His) is 486 a.a., with molecular weight of ~58 kDa.

MST1/STK4 蛋白是一种应激激活的促凋亡激酶，具有多种细胞功能，包括在 Hippo 信号通路中发挥关键作用。Caspase 裂解后，MST1/STK4 进入细胞核，诱导染色质浓缩和核小体间 DNA 断裂，从而导致细胞凋亡。在 Hippo 通路中，MST1/STK4 与其调节蛋白 SAV1 一起形成激酶级联，磷酸化并激活 LATS1/2-MOB1 复合物。这反过来又磷酸化并灭活 YAP1 癌蛋白和 WWTR1/TAZ，从而调节参与增殖、细胞死亡和细胞迁移的基因。MST1/STK4 对于抑制肝细胞增殖、抑制兼性成体肝干细胞和预防肿瘤形成至关重要。此外，它在细胞凋亡过程中的组蛋白修饰中发挥作用，磷酸化各种底物，如 FOXO3、MOBKL1A、MOBKL1B、RASSF2、TNNI3 和 FOXO1，影响多种细胞过程，如转录、细胞死亡启动和信号通路（如 PKB/AKT1 和 AR）。值得注意的是，MST1/STK4 作为 PKB/AKT1 的抑制剂，其 SIRT1 磷酸化可促进 p53 依赖性转录和细胞凋亡以响应 DNA 损伤。此外，它与 PKB/AKT1 信号传导交叉，通过拮抗 AR-染色质复合物的形成来抑制 AR 活性。

The specific activity was determined to be >150 nmol/min/mg using MBP as substrate.

Human

Sf9 insect cells

N-10*His

Q13043 (E2-F487)

6789  [NCBI]

Approximately 58 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.