Muellerian-inhibiting factor/AMH 蛋白, Mouse (P.pastoris, His)

Mullerian inhibitory factor (AMH) plays a crucial role in various reproductive processes. It contributes significantly to Mullerian duct degeneration in male fetal sex differentiation. Muellerian-inhibiting factor/AMH Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Muellerian-inhibiting factor/AMH protein, expressed by P. pastoris , with N-His labeled tag. The total length of Muellerian-inhibiting factor/AMH Protein, Mouse (P.pastoris, His) is 103 a.a., with molecular weight of ~24 kDa.

苗勒氏管抑制因子 (AMH) 蛋白在各种生殖过程中发挥着关键作用。在男性胎儿性别分化过程中，它对缪勒管退化有显着贡献。在成人中，AMH 在间质细胞分化和功能中发挥作用。相反，在女性中，AMH 充当负调节因子，阻碍原始卵泡向初级卵泡的转变，并降低生长卵泡的 FSH 敏感性。AMH 通过与其唯一的 II 型受体 AMHR2 结合来发挥作用，AMHR2 招募 I 型受体 ACVR1 和 BMPR1A，随后激活 Smad 通路。在结构上，AMH 以同二聚体形式存在，二硫键有助于其稳定性。AMH 的多种功能强调了它在协调男性和女性生殖发育和维持的关键事件中的重要参与。

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Muellerian-inhibiting factor is immobilized at 3 µg/mL(100 µL/well) can bind Recombinant Mouse MISRII. The ED₅₀ for this effect is 64.53 ng/mL.

Mouse

P. pastoris

N-His

P27106 (450D-552C)

11705  [NCBI]

DKGQDGPCALRELSVDLRAERSVLIPETYQANNCQGACRWPQSDRNPRYGNHVVLLLKMQARGAALGRLPCCVPTAYAGKLLISLSEERISADHVPNMVATEC

Approximately 24 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.