2. 重组蛋白
  3. Enzymes & Regulators
  4. N-acetylgalactosamine kinase/GALK2 蛋白, Human (HEK293, His)

N-acetylgalactosamine kinase/GALK2 蛋白, Human (HEK293, His)

目录号: HY-P70404
产品使用指南

N-乙酰半乳糖胺激酶 (GALK2) 具有双重酶作用，作用于 N-乙酰半乳糖胺 (GalNAc) 并在高半乳糖浓度下充当半乳糖激酶。GALK2 涉及潜在的挽救途径，促进复杂碳水化合物分解中游离 GalNAc 的再利用。N-acetylgalactosamine kinase/GALK2 蛋白, Human (HEK293, His) 是重组的 N-乙酰半乳糖胺激酶/GALK2 蛋白，由 HEK293 表达，带有 C-6*His 标签。

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描述

N-acetylgalactosamine kinase (GALK2) has dual enzymatic roles, acting on N-acetylgalactosamine (GalNAc) and functioning as a galactokinase under high galactose concentrations. GALK2 is implicated in a potential salvage pathway, facilitating the reutilization of free GalNAc from the breakdown of complex carbohydrates. N-acetylgalactosamine kinase/GALK2 Protein, Human (HEK293, His) is the recombinant human-derived N-acetylgalactosamine kinase/GALK2 protein, expressed by HEK293 , with C-6*His labeled tag.
研究背景

N-乙酰半乳糖胺激酶 (GALK2) 在酶活性中发挥双重作用，作用于 N-乙酰半乳糖胺 (GalNAc) 并在半乳糖浓度升高时充当半乳糖激酶。这种蛋白质涉及潜在的补救途径，有助于重新利用复杂碳水化合物分解产生的游离 GalNAc。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

Q01415-1 (M1-A458)
基因 ID
蛋白结构
N-term
GALK2 (M1-A458)
Accession # Q01415-1
6*His
C-term
同用名
rHuN-acetylgalactosamine kinase/GALK2, His; N-acetylgalactosamine kinase; GalNAc kinase; Galactokinase 2; GALK2 and GK2
氨基酸序列

MATESPATRRVQVAEHPRLLKLKEMFNSKFGSIPKFYVRAPGRVNIIGEHIDYCGYSVLPMAVEQDVLIAVEPVKTYALQLANTNPLYPDFSTSANNIQIDKTKPLWHNYFLCGLKGIQEHFGLSNLTGMNCLVDGNIPPSSGLSSSSALVCCAGLVTLTVLGRNLSKVELAEICAKSERYIGTEGGGMDQSISFLAEEGTAKLIEFSPLRATDVKLPSGAVFVIANSCVEMNKAATSHFNIRVMECRLAAKLLAKYKSLQWDKVLRLEEVQAKLGISLEEMLLVTEDALHPEPYNPEEICRCLGISLEELRTQILSPNTQDVLIFKLYQRAKHVYSEAARVLQFKKICEEAPENMVQLLGELMNQSHMSCRDMYECSCPELDQLVDICRKFGAQGSRLTGAGWGGCTVSMVPADKLPSFLANVHKAYYQRSDGSLAPEKQSLFATKPGGGALVLLEA

分子量

Approximately 47.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

N-acetylgalactosamine kinase/GALK2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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