NCAM-1/CD56 蛋白, Rat (HEK293, His)

The NCAM-1/CD56 protein is a key cell adhesion molecule that plays a key role in neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth.Its interaction with MDK contributes to complex cellular interactions in neural development.NCAM-1/CD56 Protein, Rat (HEK293, His) is the recombinant rat-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-His labeled tag.

NCAM-1/CD56 蛋白是一种细胞粘附分子，在各种神经元过程中发挥着关键作用，包括神经元-神经元粘附、神经突束颤和神经突生长。它与 MDK 相互作用，促进神经发育中复杂的细胞相互作用。此外，NCAM-1 是涉及 SLC39A6、SLC39A10 和 NCAM1 本身的复合物的一部分，该复合物调节 NCAM1 磷酸化并促进其在上皮间质转化过程中整合到粘着斑复合物中。这强调了 NCAM-1 在细胞粘附和信号转导事件中的动态参与，强调了其在神经发育和与上皮间质转化相关的细胞过程中的重要性。

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.839 μg/mL, corresponding to a specific activity is 1.19×10³ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED₅₀ this effect is 0.839 μg/mL, corresponding to a specific activity is 1.19×10³ units/mg.

Rat

HEK293

C-His

P13596 (L20-T721)

24586  [NCBI]

LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEEAWHSKWYDAKEANMEGIVTIMGLKPETRYAVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST

115-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 0.15M NaCl, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.