NETO1 蛋白, Human (HEK293, His)

The NETO1 protein complexly maintains neuronal circuits as an auxiliary subunit of NMDARs, which is critical for the abundance of GRIN2A-containing NMDARs in the postsynaptic density. It regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, particularly in spatial learning and memory. NETO1 Protein, Human (HEK293, His) is the recombinant human-derived NETO1 protein, expressed by HEK293 , with C-His labeled tag.

NETO1 蛋白错综复杂地参与神经元回路的发育和维持。NETO1 作为神经元 N-甲基-D-天冬氨酸受体 (NMDAR) 的辅助亚基，在维持突触后密度中含有 GRIN2A 的 NMDAR 丰度方面发挥着关键作用。此外，NETO1 还充当长期 NMDA 受体依赖性突触可塑性和认知的调节器，特别是在空间学习和记忆的背景下。该蛋白的相互作用通过其 C 端 TRV 结构域延伸至 DLG2、DLG3 和 DLG4 的 PLZ 结构域，突出显示其参与突触信号传导复合物。此外，NETO1 通过其 CUB 结构域与 GRIN2A 和 GRIN2B 相互作用，表明其在调节神经元突触中 NMDAR 的组成和功能中的作用。

Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED₅₀ of 0.6861 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED₅₀ of 0.6861 μg/mL.

Human

HEK293

C-6*His

Q8TDF5-3 (T23-T344)

81832  [NCBI]

TKKGTEKQTTSETQKSVQCGTWTKHAEGGIFTSPNYPSKYPPDRECIYIIEAAPRQCIELYFDEKYSIEPSWECKFDHIEVRDGPFGFSPIIGRFCGQQNPPVIKSSGRFLWIKFFADGELESMGFSARYNFTPDPDFKDLGALKPLPACEFEMGGSEGIVESIQIMKEGKATASEAVDCKWYIRAPPRSKIYLRFLDYEMQNSNECKRNFVAVYDGSSSVEDLKAKFCSTVANDVMLRTGLGVIRMWADEGSRNSRFQMLFTSFQEPPCEGNTFFCHSNMCINNTLVCNGLQNCVYPWDENHCKEKRKTSLLDQLTNTSGT

Approximately 40-50 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.