Neuregulin-1/NRG1 蛋白, Mouse (HEK293, His)

NRG1 Protein is a direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells. Binds to ERBB4 and ERBB3. Neuregulin-1/NRG1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Neuregulin-1/NRG1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Neuregulin-1/NRG1 Protein, Mouse (HEK293, His) is 227 a.a..

NRG1 Protein 与整合素和ERRB3形成的三元复合物对 NRG1-ERBB 信号传导至关重要。它诱导 MAPK3/ERK1, MAPK1/ERK2 和 AKT1的磷酸化和激活，并且配体依赖性 ERBB4 内吞作用对于 nrg1 介导的神经元中这些激酶的激活是必不可少的(。

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is 3.172 ng/mL, corresponding to a specific activity is 3.15×10⁵ units/mg.

Mouse

HEK293

C-6*His

XP_006509143 (S20-K246)

211323

SRGKPAPAEGDPSPALPPRLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRRNKPQNVKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNDLTTGMSASTERPYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLIKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAEELYQK

Approximately 42-55 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.