RTN4RL1/NgR3 蛋白, Human (HEK293, His)

The RTN4RL1/NgR3 protein is a cell surface receptor that functionally affects postnatal brain development and axonal regeneration in the adult central nervous system. It aids in axonal migration across the midline of the brain and formation of the corpus callosum. RTN4RL1/NgR3 Protein, Human (HEK293, His) is the recombinant human-derived RTN4RL1/NgR3 protein, expressed by HEK293 , with C-6*His labeled tag.

RTN4RL1/NgR3 是一种细胞表面受体，在出生后大脑发育中发挥功能冗余的作用，对成人中枢神经系统的轴突再生发挥调节控制。其贡献包括引导轴突穿过大脑中线迁移并促进胼胝体的形成。此外，RTN4RL1 还具有针对可能由 MAG 介导的运动神经元凋亡的保护功能。值得注意的是，它通过与神经元硫酸软骨素蛋白聚糖的相互作用，在抑制神经突生长和轴突再生方面发挥着至关重要的作用。该受体的结合亲和力延伸至肝素。正如在其家族对应物中观察到的那样，RTN4RL1 参与大脑发育过程中树突棘和突触数量的塑造。其信号传导能力触发 Rho 的激活，最终导致下游肌动蛋白细胞骨架重组。RTN4RL1 与 RTN4R 和 NGFR 形成复合物，依赖于硫酸软骨素蛋白聚糖的存在，同时与 MAG、OMG 和 RTN4 没有相互作用。

Human

HEK293

C-6*His

Q86UN2 (C25-A419)

146760  [NCBI]

CPRDCVCYPAPMTVSCQAHNFAAIPEGIPVDSERVFLQNNRIGLLQPGHFSPAMVTLWIYSNNITYIHPSTFEGFVHLEELDLGDNRQLRTLAPETFQGLVKLHALYLYKCGLSALPAGVFGGLHSLQYLYLQDNHIEYLQDDIFVDLVNLSHLFLHGNKLWSLGPGTFRGLVNLDRLLLHENQLQWVHHKAFHDLRRLTTLFLFNNSLSELQGECLAPLGALEFLRLNGNPWDCGCRARSLWEWLQRFRGSSSAVPCVSPGLRHGQDLKLLRAEDFRNCTGPASPHQIKSHTLTTTDRAARKEHHSPHGPTRSKGHPHGPRPGHRKPGKNCTNPRNRNQISKAGAGKQAPELPDYAPDYQHKFSFDIMPTARPKRKGKCARRTPIRAPSGVQQA

Approximately 63.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.