Neuropilin-1 蛋白, Cynomolgus (592a.a, HEK293, His)

Neuropilin-1 Protein, a transmembrane glycoprotein, regulates axon guidance, angiogenesis, neuronal development, and immune responses. Neuropilin-1 can interacts with VEGF and semaphorins, and promote the growth, survival, and self-renewal of tumors. Neuropilin-1 also acts as an entry factor and potentiate SARS-CoV-2 and Epstein-Barr virus (EBV) infectivity. Furthermore, Neuropilin-1 acts as a co-receptor for TGF-β, and enhances TGF-β activity via the SMAD2/3 signaling pathway^[1][2][3]. Neuropilin-1 Protein, Cynomolgus (592a.a, HEK293, His) is the recombinant cynomolgus-derived Neuropilin-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Neuropilin-1 Protein, Cynomolgus (592a.a, HEK293, His) is 592 a.a., with molecular weight of 80-100 kDa.

Neuropilin-1 蛋白是一种跨膜糖蛋白。在小鼠中，NRP1 在胸腺来源的 Tregs 上选择性表达，并增强免疫抑制功能。在人类中，NRP1 主要表达于浆细胞样树突状细胞 (pDC) 上，有助于启动免疫反应，此外还表达于从二级淋巴结分离的 T 调节细胞 (Treg) 子集上^[1]。
Neuropilin-1 转导 VEGF 以及 VEGFR 家族成员的信号。Neuropilin-1 与 VEGF 和信号蛋白相互作用，可以促进肿瘤的生长、存活和自我更新。例如，Neuropilin-1 可与血管内皮细胞中的 VEGFR2 复合，诱导血管通透性和血管生成^[3]。
Neuropilin-1 还充当进入因子并增强 SARS-CoV-2 的感染性。此外，Neuropilin-1 是 Epstein-Barr 病毒 (EBV) 感染鼻咽上皮细胞的受体，并增强 EBV 感染^[2][3]。Neuropilin-1 通过与 Semaphorin-3A (一种在神经系统和脉管系统中广泛存在的蛋白质) 相互作用，在轴突引导和修剪中发挥核心作用^[3]。
此外，Neuropilin-1 作为 TGF-β 的共同受体，并通过 SMAD2/3 信号通路增强 TGF-β 活性。Neuropilin-1 可以激活非活性潜伏形式 (LAP-TGF-β)，并促进免疫抑制^[1]。
Neuropilin-1 在血管生成、神经元发育和免疫反应调节中发挥着重要作用。

Cynomolgus

HEK293

C-6*His

G7PEQ1 (F22-G613)

102145235  [NCBI]

FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGRLWGKFCGKIAPPPVVSSGQFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEAVGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDISSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISLRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYVNEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNPVDECDDDQANCHSG

80-100 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.