Neuropilin-1 蛋白, Human (HEK293, Fc)

Neuropilin-1 protein plays an important role by binding to VEGF-165, possibly inhibiting its cellular binding and inducing apoptosis. This dual function suggests its role in regulating VEGF-mediated processes. Neuropilin-1 Protein, Human (HEK293, Fc) is the recombinant human-derived Neuropilin-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Neuropilin-1 Protein, Human (HEK293, Fc) is 623 a.a., with molecular weight of 116-130 kDa.

Neuropilin-1 蛋白在与 VEGF-165 结合时发挥着重要作用，可能抑制其与细胞的结合，并通过隔离 VEGF-165 诱导细胞凋亡。这种双重功能表明 Neuropilin-1 参与 VEGF 介导的细胞过程的调节。此外，Neuropilin-1 可能与信号蛋白家族的各个成员相互作用，这表明它在与不同配体结合方面具有多功能性。值得注意的是，它的表达似乎对血管数量和完整性产生不利影响，表明其在血管生成和血管发育中具有潜在作用。Neuropilin-1 的多种相互作用和调节作用强调了它在协调复杂细胞过程中的重要性及其在血管生成和细胞凋亡途径中的潜在影响。

Neuropilin-1 (1 nM；15 min) 激活 MDA-MB-231 细胞表面的 LAP-TGF-β1 (1.4 nM)，并与其共内化^[1]。

1. Immobilized Human VEGF165 at 2 μg/mL (100 μL/well) can bind Human NRP1 Fc with a linear range of 0.2-4 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized Neuropilin-1 at 2 μg/ml (100 μl/well) can bind Biotinylated human VEGF165. The ED₅₀ for this effect is 0.01063 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Neuropilin-1 at 2 μg/ml (100 μl/well) can bind Biotinylated human VEGF165.The ED₅₀ for this effect is 0.01063 μg/mL.

Human

HEK293

C-hFc

AAH07533 (F22-K644)

8829  [NCBI]

FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK

116-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 orPBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Glinka Y, et al. Neuropilin-1 exerts co-receptor function for TGF-beta-1 on the membrane of cancer cells and enhances responses to both latent and active TGF-beta. Carcinogenesis. 2011 Apr;32(4):613-21.  [Content Brief]