Neurotrophin-3 蛋白, Human

Neurotrophin-3 Protein, Human is a recombinant Neurotrophin-3 protein expressed in E. coli system. Neurotrophin-3 is widely expressed in the nervous system. Neurotrophin-3 reduces cellular damage, improves neuronal regeneration in different models of lesions^[1].

神经营养蛋白-3 (NT-3) 是神经营养蛋白家族的一种营养因子，广泛表达于神经系统中。
NT-3 的生理作用由两种膜受体的激活介导：低亲和力的膜受体受体p75和高亲和力受体Trk。NT-3 激活 TrkC 受体，并与 TrkB 和 TrkA 结合，亲和力较小。
NT-3 减少细胞损伤，改善不同病变或神经变性模型中的神经元再生，参与突触重组、突触形成和神经元再生。可塑性^[1]。

Neurotrophin-3 (人; 2 ng/mL; 20 h) 而不是脑源性神经营养因子，促进视网膜祖细胞 E4 的神经元分化^[3]。

1.The ED₅₀ as determined by its ability to bind Human NTRK2 in functional ELISA is less than 10 μg/mL.
2.Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 35.88 ng/mL, corresponding to a specific activity is 2.787×10⁴ units/mg.

Human

E. coli

Tag Free

P20783-1 (Y139-T257)

4908  [NCBI]

YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Approximately 14-15 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2 or 0.1% TFA, 20% Acetonitrile, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Koichi Uegaki, et al. BDNF Binds Its Pro-Peptide with High Affinity and the Common Val66 Met Polymorphism Attenuates the Interaction. Int J Mol Sci. 2017 May 12;18(5):1042.  [Content Brief]

  [2]. O Blondel, et al. A glia-derived signal regulating neuronal differentiation. J Neurosci. 2000 Nov 1;20(21):8012-20.  [Content Brief]

  [3]. de la Rosa EJ, et al. Role of neurotrophins in the control of neural development: neurotrophin-3 promotes both neuron differentiation and survival of cultured chick retinal cells. Neuroscience. 1994 Jan;58(2):347-52.  [Content Brief]