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  3. NIP7 蛋白, Human (His)

NIP7 蛋白, Human (His)

目录号: HY-P71162
产品使用指南

NIP7 对于 34S pre-rRNA 的精确加工和 60S 核糖体亚基的组装至关重要。作为单体,NIP7 与前核糖体复合物相互作用,可能与 RNA 结合,并与关键蛋白伴侣结合,包括 NOL8、SBDS 和 FTSJ3。NIP7 蛋白, Human (His) 是重组的 NIP7 蛋白,由 E. coli 表达,带有 N-6*His 标签。

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描述

NIP7 is essential for precise processing of 34S pre-rRNA and assembly of 60S ribosomal subunits. As a monomer, NIP7 interacts with preribosomal complexes, may bind to RNA, and binds to key protein partners, including NOL8, SBDS, and FTSJ3. NIP7 Protein, Human (His) is the recombinant human-derived NIP7 protein, expressed by E. coli , with N-6*His labeled tag.

研究背景

NIP7在确保34S pre-rRNA的精确加工和60S核糖体亚基的组装方面发挥着至关重要的作用。NIP7 作为单体运行,与前核糖体复合物相互作用,可能与 RNA 结合,并与各种蛋白质伙伴(例如 NOL8、SBDS 和 FTSJ3)进行重要的相互作用。这些相互作用促成了核糖体亚基成熟和组装所涉及的复杂分子事件网络,突出了 NIP7 在核糖体生物发生的复杂机制中的重要性。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9Y221-1 (M1-T180)

基因 ID
蛋白结构
N-term
6*His
NIP7 (M1-T180)
Accession # Q9Y221-1
C-term
同用名
60S Ribosome Subunit Biogenesis Protein NIP7 Homolog; KD93; NIP7
氨基酸序列

MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEKIMKLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWIKPGAEQSFLYGNHVLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKSTQDCRKVDPMAIVVFHQADIGEYVRHEETLT

分子量

Approximately 20.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NIP7 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NIP7 Protein, Human (His)
目录号:
HY-P71162
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