NKp46/NCR1 蛋白, Human (HEK293, His)

NKp46/NCR1 Protein is a major NK cell-activating receptor that is involved in the elimination of target cells and recognizing a wide range of tumors, viruses, and bacteria. NKp46 forms microclusters structures at the immune synapse between NK cells and target cells. Over-expression of human NKp46 is correlated with increased accumulation of F-actin mesh at the immune synapse. NKp46 signaling directly regulates the NK lytic immune synapse from early formation to late function^[1][2][3]. NKp46/NCR1 Protein, Human (HEK293, His) is the recombinant human-derived NKp46/NCR1 protein, expressed by HEK293 , with N-His labeled tag. The total length of NKp46/NCR1 Protein, Human (HEK293, His) is 233 a.a., with molecular weight of ~27.5 kDa.

NKp46 是一种参与天然细胞毒性的新型触发受体。NKp46 是一种约为 ~46 kDa 1 型跨膜糖蛋白，其特征在于细胞内尾部具有 30 个氨基酸，跨膜结构域具有 20 个氨基酸和两个通过 25 个氨基酸短肽连接的细胞外 Ig 样结构域^[1]。
NKp46 在所有 NK 细胞亚群上都有独特表达，并被建议作为 NK 细胞消融的可能靶标和泛 NK 细胞标记物。与 NCR 不同，NKp46 (NCR1) 在小鼠和人类之间进化保守。在原代人 NK 细胞中敲除 NKp46 会减少 F-肌动蛋白向突触的募集^[1]。
NKp46 通过与受感染的肺泡巨噬细胞相互作用，有助于清除肺炎链球菌。使用 NKp46 抗体靶向 NK 细胞可以减缓小鼠 1 型糖尿病的进展。NKp46 还调节移植物抗宿主病和过敏反应。NK 与靶细胞相互作用开始后，NKp46 聚集在细胞膜上，特别是免疫突触处。在免疫突触处，NKp46 介导细胞骨架重排和细胞极化^[1]。
NKp46 的交联导致 NK 细胞强烈活化，从而诱导 Ca²⁺ 动员、细胞毒性和淋巴因子释放^[2]。
天然细胞毒性受体 (NCR) 家族包括 NKp30、NKp44 和 NKp46，是激活人类 NK 细胞受体的最大家族。NKp46 是唯一具有小鼠直系同源物 (名为 Ncr1) 的 NCR 成员。第一个被鉴定为 NKp46/NCR1 受体的配体是流感病毒的血凝素 (HA) 蛋白^[3]。

Measured by its binding ability in a functional ELISA. When immobilized Human NKp46 at 0.5 μg/mL (100μL/Well) on the plate, it binds Anti-NKp46 Ab with an EC₅₀ of 17.7 ng/mL.

Human

HEK293

N-His

O76036-6/AAH64806 (Q22-D254)

9437  [NCBI]

Approximately 27.5 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.