NNMT 蛋白, Human (GST)

The nicotinamide N-methyltransferase (NNMT) protein catalyzes the methylation of nicotinamide using S-adenosyl-L-methionine to form N1-methylnicotinamide. NNMT affects pluripotent embryonic stem cell development and acts as a metabolic regulator, affecting adipose tissue energy expenditure, gluconeogenesis, and cholesterol biosynthesis. NNMT Protein, Human (GST) is the recombinant human-derived NNMT protein, expressed by E. coli , with N-GST labeled tag. The total length of NNMT Protein, Human (GST) is 264 a.a., with molecular weight of 56.6 kDa.

烟酰胺 N-甲基转移酶 (NNMT) 负责催化烟酰胺的 N-甲基化，利用通用甲基供体 S-腺苷-L-甲硫氨酸产生 N1-甲基烟酰胺和 S-腺苷-L-高半胱氨酸，代表烟酰胺的重要途径/维生素B3清除。这种酶活性通过消耗 S-腺苷-L-甲硫氨酸在细胞甲基化电位调节中发挥核心作用，从而限制了其对其他甲基转移酶的利用。NNMT 通过抑制性染色质标记（例如 H3K27me3）的低甲基化，积极协调全基因组表观遗传和转录变化，并有助于在发育过程中多能胚胎干细胞植入前状态中建立低水平的抑制性组蛋白标记。从功能上讲，NNMT 作为代谢调节剂，影响白色脂肪组织的能量消耗、肝脏糖异生和胆固醇生物合成。在白色脂肪细胞中，它通过补救途径调节多胺通量并控制 NAD(+) 水平。此外，NNMT 通过产生 N1-甲基烟酰胺，影响肝细胞中的蛋白质乙酰化，抑制泛素化并增强 SIRT1 脱乙酰酶的稳定性。此外，NNMT 通过 N-甲基化与烟酰胺结构相关的其他吡啶而表现出多功能性，表明其在异生物质解毒中发挥作用。

Human

E. coli

N-GST

P40261 (M1-L264)

4837

56.6 kDa

Greater than 90 % as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.