Norrin 蛋白, Mouse

Norrin protein is a key component of the canonical Wnt signaling pathway, activating the cascade by interacting with the FZD4 receptor and LRP5 coreceptor. Its role in retinal vascularization includes serving as a FZD4 ligand, stabilizing β-catenin (CTNNB1), and initiating LEF/TCF-mediated transcription. Norrin Protein, Mouse is the recombinant mouse-derived Norrin protein, expressed by E. coli , with tag free. The total length of Norrin Protein, Mouse is 107 a.a., with molecular weight of ~13 kDa.

Norrin 蛋白是经典 Wnt 信号通路中的关键参与者，通过与 FZD4 受体和 LRP5 辅助受体的相互作用激活该级联。它在视网膜血管化中的核心作用是通过充当 FZD4 的配体来协调的，从而稳定 β-连环蛋白 (CTNNB1) 并启动 LEF/TCF 介导的转录程序。Norrin 与 TSPAN12 合作，展示了独立于经典 Wnt 信号传导激活 FZD4 的独特能力，表明存在另一种不依赖于 Wnt 的途径，该途径也有助于 β-catenin 的积累。除了参与视网膜血管化之外，Norrin 还可能参与影响神经细胞分化和增殖的更广泛的调节网络，以及在神经外胚层细胞间相互作用中的潜在作用。在结构上，Norrin 形成通过二硫键连接的同二聚体，并且是包含 TSPAN12、FZD4、LRP5/6 及其自身的更大复合物的一部分，突出了其在细胞信号传导景观中的多方面相互作用。

Measured by its binding ability in a functional ELISA. When Mouse Norrin is coated at 0.5 μg/mL (100 μL/well) can bind Human Frizzled­4. The ED₅₀ for this effect is 84.14 ng/mL.

Mouse

E. coli

Tag Free

P48744 (K25-S131)

17986  [NCBI]

KTDSSFLMDSQRCMRHHYVDSISHPLYKCSSKMVLLARCEGHCSQASRSEPLVSFSTVLKQPFRSSCHCCRPQTSKLKALRLRCSGGMRLTATYRYILSCHCEECSS

Approximately 13 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 4 mM HCL, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.