1. 重组蛋白
  2. Receptor Proteins Biotinylated Proteins
  3. Notch family
  4. Notch-3
  5. Notch 3 蛋白, Human (HEK293, C-His-Avi)

Notch 3 蛋白, Human (HEK293, C-His-Avi)

目录号: HY-P700804
产品使用指南

Notch 3 蛋白是配体 JAG1、JAG2 和 DLL1 的受体,在细胞命运决定中发挥着至关重要的调节作用。配体激活后,NICD 与 RBPJ/RBPSUH 形成转录激活剂复合物,并激活切割位点增强子内的基因。Notch 3 蛋白, Human (HEK293, C-His-Avi) 是重组的 Notch 3 蛋白,由 HEK293 表达,带有 C-His 标签。Notch 3 蛋白, Human (HEK293, C-His-Avi) 全长 263 个氨基酸,分子量分别为 27-30 kDa & 10-12 kDa。

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描述

Notch 3 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and plays a crucial regulatory role in cell fate determination. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes within the enhancer of the cleavage site. Notch 3 Protein, Human (HEK293, C-His-Avi) is the recombinant human-derived Notch 3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Notch 3 Protein, Human (HEK293, C-His-Avi) is 263 a.a., with molecular weight of 27-30 kDa & 10-12 kDa, respectively.

研究背景

Notch 3 蛋白作为膜结合配体(包括 Jagged1、Jagged2 和 Delta1)的受体,在调节细胞命运决定中发挥着关键作用。配体激活后,释放的缺口胞内结构域 (NICD) 与 RBPJ/RBPSUH 形成转录激活剂复合物,启动分裂位点增强子内基因的激活。这种多方面的蛋白质影响细胞分化、增殖和凋亡程序。在结构上,它以异二聚体形式存在,由 C 端片段 (N(TM)) 和 N 端片段 (N(EC)) 组成,可能通过二硫键连接。Notch 3 与转录共激活因子 MAML1、MAML2 和 MAML3 相互作用,调节下游转录过程。它还与 PSMA1 和 HIF1AN 结合,促进多种细胞功能和调节途径。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q9UM47 (A1378-S1640)

基因 ID
蛋白结构
N-term
Notch 3 (A1378-S1640)
Accession # Q9UM47
His
C-term
同用名
CADASIL; Notch homolog 3; Notch-3; NOTCH3
分子量

27-30 kDa & 10-12 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Notch 3 Protein, Human (HEK293, C-His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Notch 3 Protein, Human (HEK293, C-His-Avi)
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HY-P700804
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