NRROS 蛋白, Human (Baculovirus, His-Myc)

The NRROS protein is a key TGFB1 regulator with crucial effects on microglial function in the nervous system. It activates latent TGFB1 in macrophages and microglia by forming disulfide bonds with LAP, coordinating integrin-dependent TGFB1 activation. NRROS Protein, Human (Baculovirus, His-Myc) is the recombinant human-derived NRROS protein, expressed by Sf9 insect cells , with N-10*His, C-Myc labeled tag. The total length of NRROS Protein, Human (Baculovirus, His-Myc) is 632 a.a., with molecular weight of ~73.5 kDa.

NRROS 是转化生长因子 β-1 (TGFB1) 的关键调节因子，在神经系统内的小胶质细胞功能中发挥着至关重要的作用。其对小胶质细胞活性的具体要求涉及巨噬细胞和小胶质细胞中潜在 TGFB1 的激活，这是通过与 TGFB1 调节链——潜伏相关肽 (LAP) 形成二硫键来实现的。NRROS 协调整合素依赖性的 TGFB1 激活，导致高度局部化的 TGF-β-1 激活，且有限地扩散到邻近的小胶质细胞。这种局部选择性激活机制表明 LRRC33/NRROS 在调节 TGFB1 信号传导方面的精确性。此外，NRROS 间接参与 Toll 样受体 (TLR) 信号传导，抑制 TLR 介导的 NF-κ-B 激活和细胞因子产生，这可能是其在 TGF-β-1 信号传导中的作用的结果。NRROS 通过与 LAP 的二硫键与 TGFB1 相互作用，并与 TLR2、TLR3、TLR4、TLR9 和潜在的其他 Toll 样受体结合，进一步强调了其在免疫调节中的多方面参与。此外，NRROS 直接与 CYBB/NOX2 相互作用，突出了其多样化的功能关联。

Human

Sf9 insect cells

N-10*His;C-Myc

Q86YC3 (W19-L650)

375387  [NCBI]

WRNRSGTATAASQGVCKLVGGAADCRGQSLASVPSSLPPHARMLTLDANPLKTLWNHSLQPYPLLESLSLHSCHLERISRGAFQEQGHLRSLVLGDNCLSENYEETAAALHALPGLRRLDLSGNALTEDMAALMLQNLSSLRSVSLAGNTIMRLDDSVFEGLERLRELDLQRNYIFEIEGGAFDGLAELRHLNLAFNNLPCIVDFGLTRLRVLNVSYNVLEWFLATGGEAAFELETLDLSHNQLLFFPLLPQYSKLRTLLLRDNNMGFYRDLYNTSSPREMVAQFLLVDGNVTNITTVSLWEEFSSSDLADLRFLDMSQNQFQYLPDGFLRKMPSLSHLNLHQNCLMTLHIREHEPPGALTELDLSHNQLSELHLAPGLASCLGSLRLFNLSSNQLLGVPPGLFANARNITTLDMSHNQISLCPLPAASDRVGPPSCVDFRNMASLRSLSLEGCGLGALPDCPFQGTSLTYLDLSSNWGVLNGSLAPLQDVAPMLQVLSLRNMGLHSSFMALDFSGFGNLRDLDLSGNCLTTFPRFGGSLALETLDLRRNSLTALPQKAVSEQLSRGLRTIYLSQNPYDCCGVDGWGALQHGQTVADWAMVTCNLSSKIIRVTELPGGVPRDCKWERLDLGL

Approximately 73.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.