NRXN3/Neurexin-3 蛋白, Human (HEK293, His)

Neurexin-3-beta is neuronal cell surface protein that is involved in cell recognition and cell adhesion. Neurexin-3-beta mediates intracellular signaling. NRXN3/Neurexin-3 Protein, Human (HEK293, His) is the recombinant human-derived NRXN3/Neurexin-3 protein, expressed by HEK293 , with C-His labeled tag. The total length of NRXN3/Neurexin-3 Protein, Human (HEK293, His) is 322 a.a., with molecular weight of 50-60 kDa.

NRXN3 编码一个蛋白家族成员，在神经系统中作为受体和细胞粘附分子发挥作用。大量的选择性剪接和选择性启动子的使用导致该基因的多种转录变异体和蛋白质亚型，但其中许多变异体的全长性质尚未确定。上游启动子启动的转录物编码 α 亚型，其中包含表皮生长因子样 (EGF-like) 序列和层粘连蛋白 G 结构域。起始于下游启动子的转录物编码缺乏 EGF 样序列的 β 亚型。该位点的遗传变异与一系列行为表型相关，包括酒精依赖和孤独症谱系障碍^{[1][2][3][4][5]}。

Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5×10⁴ cells/well are added to NRXN3 coated plates (0.8 μg/mL and 100 μL/well), approximately 46.72% will adhere specifically after 60 minutes at 37°C.

Human

HEK293

C-His

Q9HDB5-1 (S36-L357)

9369  [NCBI]

SSNVASSSSTSSSPGSHSQHEHHFHGSKHHSVPISIYRSPVSLRGGHAGATYIFGKSGGLILYTWPANDRPSTRSDRLAVGFSTTVKDGILVRIDSAPGLGDFLQLHIEQGKIGVVFNIGTVDISIKEERTPVNDGKYHVVRFTRNGGNATLQVDNWPVNEHYPTGRQLTIFNTQAQIAIGGKDKGRLFQGQLSGLYYDGLKVLNMAAENNPNIKINGSVRLVGEVPSILGTTQTTSMPPEMSTTVMETTTTMATTTTRKNRSTASIQPTSDDLVSSAECSSDDEDFVECEPSTGGELVIPLLVEDPLATPPIATRAPSITL

Approximately 50-70 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.