NS5 蛋白, Dengue virus 2 (His)

NS5 Protein is part of the flavivirus RNA replication complex (RC) composed of viral non-structural proteins and host-cell cofactors. NS5 is the largest flavivirus protein, the most conserved, which act as two domains, the RNA-dependent RNA polymerase (RdRp) and RNA methyltransferase enzyme (MTase). NS5 plays a fundamental role in flavivirus replication, viral RNA methylation, RNA polymerization, and host immune system evasion. NS5 Protein, Dengue virus 2 (His) is the recombinant Virus-derived NS5 protein, expressed by E. coli , with N-His labeled tag. The total length of NS5 Protein, Dengue virus 2 (His) is 296 a.a., with molecular weight of ~35.5 kDa.

非结构蛋白 5 (NS5) 是黄病毒 RNA 复制复合物 (RC) 的一部分，由病毒非结构蛋白和宿主细胞辅助因子组成。NS5 是最大、最保守的黄病毒蛋白，它充当两个结构域：RNA 依赖性 RNA 聚合酶 (RdRp) 和 RNA 甲基转移酶 (MTase)。DENV-NS5 RdRp 结构域有助于病毒复制阶段，而 MTase 则启动病毒 RNA 加帽并促进多蛋白翻译。NS5 在病毒 RNA 甲基化、RNA 聚合和宿主免疫系统逃避中发挥着重要作用。它作为支架蛋白发挥作用，对宿主 STAT2 和 ERC1 蛋白具有不同的结合位点，但都需要 UBR4。NS5 还与 hSTAT2 等宿主细胞蛋白相互作用，有助于逃避 I 型干扰素 (IFN) 介导的先天免疫反应，这是宿主细胞防御病毒感染的第一步。NS5 是 DENV 蛋白 I 型 IFN 信号传导的有效拮抗剂，通过抑制人 JAK-STAT 信号传导^[1][2][3]。

Virus

E. coli

N-His

AAC59275.1 (G2492-Q2787)

/

GTGNIGETLGEKWKSRLNALGKSEFQIYKKSGIQEVDRTLAKEGIKRGETDHHAVSRGSAKLRWFVERNMVTPEGKVVDLGCGRGGWSYYCGGLKNVREVKGLTKGGPGHEEPIPMSTYGWNLVRLQSGVDVFFTPPEKCDTLLCDIGESSPNPTVEAGRTLRVLNLVENWLNNNTQFCIKVLNPYMPSVIEKMEALQRKYGGALVRNPLSRNSTHEMYWVSNASGNIVSSVNMISRMLINRFTMRHKKATYEPDVDLGSGTRNIGIESEIPNLDIIGKRIEKIKQEHETSWHYDQ

Approximately 35.5 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.