Nucleocapsid 蛋白 (His)

Nucleocapsid Protein (His) expressed by E. coli systems. Nucleocapsid Protein is required for coronavirus RNA synthesis and has RNA chaperone activity^[1].

冠状病毒是具有正义RNA基因组的有包膜病毒，并具有螺旋对称的核衣壳。在病毒感染的原代细胞和用表达 N 蛋白的质粒转染的细胞中，核衣壳蛋白定位于细胞质和核仁^[1]。
核衣壳蛋白是一种碱性蛋白，是冠状病毒中含量丰富，对核酸（包括 ssRNA、ssDNA 和 dsDNA）具有非特异性结合活性。核衣壳蛋白是人类冠状病毒 (CoV) 感染细胞中必需的 RNA 结合病毒蛋白，是病毒 RNA 复制和转录所必需的。人类 CoV NP 是抗病毒药物研究的有效靶点^[1][2]。

Immobilized 2019-nCoV NP Antibody(6G9)-Fc at 5 μg/mL(100 μl/well) can bind 2019-nCoV Nucleocapsid Protein-His. The ED₅₀ of 2019-nCoV Nucleocapsid Protein-His is 0.1-0.7 μg/mL.

Virus

E. coli

N-6*His

QHD43423.2 (M1-A419)

43740575  [NCBI]

HHHHHHMSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA

50-60 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 300 mM NaCl, 10% Glycerol, 1 mM EDTA, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Sander van Boheemen, et al. Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans. mBio. 2012 Nov 20;3(6):e00473-12.  [Content Brief]

  [2]. Chung-ke Chang, et al. Structure-based virtual screening and experimental validation of the discovery of inhibitors targeted towards the human coronavirus nucleocapsid protein. Mol Biosyst. 2016 Jan;12(1):59-66.  [Content Brief]