Nucleoprotein/NP 蛋白, Influenza B virus (His-SUMO)

Nucleoprotein (NP) protects viral RNA and serves as a replication template. Nucleoprotein/NP Protein, Influenza B virus (His-SUMO) is the recombinant Virus-derived Nucleoprotein/NP protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Nucleoprotein/NP Protein, Influenza B virus (His-SUMO) is 560 a.a., with molecular weight of ~78.0 kDa.

核蛋白/NP 蛋白通过包裹负链病毒 RNA、保护其免受核酸酶侵害并形成核糖核蛋白 (RNP) 复合物，在流感病毒生命周期中发挥着关键作用。该 RNP 充当转录和复制过程的模板。为了启动感染周期，RNP 必须定位在宿主细胞核中，这一任务因其大小而变得复杂，防止通过核孔复合体扩散。NP 拥有至少两个核定位信号，促进活性 RNP 通过细胞导入 α/β 途径导入细胞核。在感染后期，RNP 的核输出是由病毒蛋白 NEP 与 M1 相互作用精心策划的，M1 结合核蛋白。核蛋白有可能直接与宿主exportin-1/XPO1结合，积极参与RNPs核输出。M1 与 RNP 的相互作用隐藏了核蛋白的核定位信号，但在 M2 蛋白驱动的病毒粒子酸化后，M1 从 RNP 解离，揭示核蛋白的信号并将 RNP 引导至细胞核。核蛋白同源多聚化形成核衣壳并可直接结合宿主输出蛋白-1/XPO1。复杂的蛋白质-RNA 接触涉及带正电残基和磷酸主链之间的静电相互作用，以及芳香族侧链和碱基之间的平面相互作用。

Virus

E. coli

N-SUMO;N-6*His

P04666 (M1-Y560)

/

MSNMDIDGINTGTIDKTPEEIISGTSGATRPIIRPATLAPPSNKRTRNPSPERATTSSEADVGRKTQKKQTPTEIKKSVYNMVVKLGEFYNQMMVKAGLNDDMERNLIQNAHAVERILLAATDDKKTEFQKKKNARDVKEGKEEIDHNKTGGTFYKMVRDDKTIYFSPIRITFLKEEVKTMYKTTMGSDGFSGLNHIMIGHSQMNDVCFQRSKALKRVGLDPSLISTFAGSTLPRRSGATGVAIKGGGTLVAEAIRFIGRAMADRGLLRDIKAKTAYEKILLNLKNKCSAPQQKALVDQVIGSRNPGIADIEDLTLLARSMVVVRPSVASKVVLPISIYAKIPQLGFNVEEYSMVGYEAMALYNMATPVSILRMGDDAKDKSQLFFMSCFGAAYEDLRVLSALTGTEFKPRSALKCKGFHVPAKEQVEGMGAALMSIKLQFWAPMTRSGGNEVGGDGGSGQISCSPVFAVERPIALSKQAVRRMLSMNIEGRDADVKGNLLKMMNDSMAKKTNGNAFIGKKMFQISDKNKTNPVEIPIKQTIPNFFFGRDTAEDYDDLDY

Approximately 78.0 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.