Nucleoprotein/NP 蛋白, Influenza A virus H1N1 (His-SUMO)

Nucleoprotein/NP Protein, a key component of the influenza virus, plays a vital role in viral replication and transcription. It binds to viral RNA, forming a ribonucleoprotein complex essential for viral genome replication. NP Protein also interacts with host proteins, contributing to viral pathogenesis and immune evasion. Understanding the functions of NP Protein can aid in the development of antiviral strategies against influenza infections. Nucleoprotein/NP Protein, Influenza A virus H1N1 (His-SUMO) is the recombinant Virus-derived Nucleoprotein/NP protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Nucleoprotein/NP Protein, Influenza A virus H1N1 (His-SUMO) is 498 a.a., with molecular weight of ~72.1 kDa.

核蛋白 (NP) 蛋白通过包裹负链病毒 RNA，提供针对核酸酶的保护，在病毒复制中发挥着至关重要的作用。这种衣壳化的基因组 RNA 形成核糖核蛋白 (RNP)，充当转录和复制的模板。为了启动感染周期，RNP 需要位于宿主细胞核中，但其较大的尺寸阻止了通过核孔复合体的扩散。NP 包含两个核定位信号，可主动促进 RNP 通过细胞导入 α/β 途径导入细胞核。在感染后期，RNP 的核输出由病毒蛋白 NEP 介导，NEP 与 M1 相互作用，与核蛋白结合。核蛋白有可能直接与宿主exportin-1/XPO1结合并积极参与RNPs核输出。M1 与 RNP 的相互作用似乎隐藏了核蛋白的核定位信号。然而，在感染新细胞后，由于 M2 蛋白驱动的病毒体酸化，M1 与 RNP 分离。这种解离揭示了核蛋白的核定位信号，导致目标 RNP 转运到细胞核中。此外，核蛋白形成同源多聚体以产生核衣壳，并可能与宿主输出蛋白-1/XPO1 结合。蛋白质-RNA相互作用涉及带正电残基和磷酸主链之间的静电相互作用以及芳香族侧链和碱基之间的平面相互作用的组合。

Virus

E. coli

N-SUMO;N-6*His

P18071 (M1-N498)

/

MASQGTKRSYEQMETDGDRQNATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYKRVDGKWMRELVLYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELIRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGPAVASGYDFEKEGYSLVGIDPFKLLQNSQVYSLIRPNENPAHKSQLVWMACNSAAFEDLRVSSFIRGTKVIPRGKLSTRGVQIASNENMDTMGSSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPFDKTTIMAAFTGNAEGRTSDMRAEIIRMMESARPEEVSFQGRGVFELSDERAANPIVPSFDMSNEGSYFFGDNAEEYDN

Approximately 72.1kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<17 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.