OLFM4 蛋白, Human (HEK293, His)

OLFM4 protein may promote the proliferation of pancreatic cancer cells by affecting the transition from S phase to G2/M phase, highlighting its role in cancer cell cycle dynamics. In myeloid leukemia cell lines, OLFM4 exhibits dual effects of inhibiting growth and inducing differentiation and apoptosis. OLFM4 Protein, Human (HEK293, His) is the recombinant human-derived OLFM4 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of OLFM4 Protein, Human (HEK293, His) is 490 a.a., with molecular weight of 60-90 kDa.

OLFM4 蛋白通过促进 S 期向 G2/M 期的转变，可能促进胰腺癌细胞的增殖，强调了其在癌细胞细胞周期动力学中的潜在作用。相反，在髓系白血病细胞系中，OLFM4 抑制细胞生长，同时诱导细胞分化和凋亡，表明其在不同的细胞环境中发挥双重作用。此外，它可能有助于抑制 EIF4EBP1 磷酸化/失活，表明其在调节蛋白质翻译过程中具有潜在作用。已知 OLFM4 可能通过与细胞表面凝集素和钙粘蛋白的相互作用来促进细胞粘附。在结构上，OLFM4 作为具有二硫键的同多聚体存在。它与 NDUFA13 相互作用，并对细胞表面凝集素表现出结合亲和力，包括蓖麻凝集素 I、伴刀豆球蛋白-A、麦芽凝集素以及钙粘蛋白。OLFM4 的多方面功能和相互作用凸显了其在细胞过程中的重要性，对癌症进展和细胞粘附具有影响。进一步的研究对于揭示 OLFM4 调节多种细胞功能的精确分子机制至关重要。

Measured by the ability of the immobilized protein to support the spreading of NIH3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.625-1.25 μg/mL, corresponding to a specific activity is 800-1600 U/mg.

Human

HEK293

C-10*His

Q6UX06 (D21-Q510)

10562  [NCBI]

DLGDVGPPIPSPGFSSFPGVDSSSSFSSSSRSGSSSSRSLGSGGSVSQLFSNFTGSVDDRGTCQCSVSLPDTTFPVDRVERLEFTAHVLSQKFEKELSKVREYVQLISVYEKKLLNLTVRIDIMEKDTISYTELDFELIKVEVKEMEKLVIQLKESFGGSSEIVDQLEVEIRNMTLLVEKLETLDKNNVLAIRREIVALKTKLKECEASKDQNTPVVHPPPTPGSCGHGGVVNISKPSVVQLNWRGFSYLYGAWGRDYSPQHPNKGLYWVAPLNTDGRLLEYYRLYNTLDDLLLYINARELRITYGQGSGTAVYNNNMYVNMYNTGNIARVNLTTNTIAVTQTLPNAAYNNRFSYANVAWQDIDFAVDENGLWVIYSTEASTGNMVISKLNDTTLQVLNTWYTKQYKPSASNAFMVCGVLYATRTMNTRTEEIFYYYDTNTGKEGKLDIVMHKMQEKVQSINYNPFDQKLYVYNDGYLLNYDLSVLQKPQ

60-90 kDa

• 
  Greater than 80% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.