OPRD1 蛋白, Human (Cell-Free, His)

The OPRD1 protein is a G protein-coupled receptor for enkephalins and opioids that undergoes ligand-induced conformational changes to activate signaling through G proteins and inhibit adenylate cyclase. Additionally, it reduces neurotransmitter release by affecting ionic currents. OPRD1 Protein, Human (Cell-Free, His) is the recombinant human-derived OPRD1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of OPRD1 Protein, Human (Cell-Free, His) is 372 a.a., with molecular weight of 41.9 kDa.

OPRD1 蛋白是一种 G 蛋白偶联受体，可作为内源性脑啡肽和其他阿片类药物子集的受体。配体结合后，受体会发生构象变化，通过鸟嘌呤核苷酸结合蛋白（G 蛋白）启动信号传导并调节腺苷酸环化酶等下游效应子，从而抑制腺苷酸环化酶活性。此外，OPRD1 通过减少钙离子电流和增加钾离子电导来抑制神经递质释放。至关重要的是，它在疼痛感知和阿片介导的镇痛中发挥着关键作用，有助于吗啡镇痛耐受的发展。OPRD1可以形成同源寡聚体并与OPRM1形成异二聚体，与GPRASP1和RTP4相互作用以调节OPRD1-OPRM1异二聚体的细胞表面定位。这些相互作用凸显了 OPRD1 调节阿片类信号传导和镇痛反应的复杂调节机制。

Human

E. coli Cell-free

N-10*His

P41143 (M1-A372)

4985

MEPAPSAGAELQPPLFANASDAYPSACPSAGANASGPPGARSASSLALAIAITALYSAVCAVGLLGNVLVMFGIVRYTKMKTATNIYIFNLALADALATSTLPFQSAKYLMETWPFGELLCKAVLSIDYYNMFTSIFTLTMMSVDRYIAVCHPVKALDFRTPAKAKLINICIWVLASGVGVPIMVMAVTRPRDGAVVCMLQFPSPSWYWDTVTKICVFLFAFVVPILIITVCYGLMLLRLRSVRLLSGSKEKDRSLRRITRMVLVVVGAFVVCWAPIHIFVIVWTLVDIDRRDPLVVAALHLCIALGYANSSLNPVLYAFLDENFKRCFRQLCRKPCGRPDPSSFSRAREATARERVTACTPSDGPGGGAAA

Monomer: 39 kDa Dimer: 78 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution PBS, 0.05% Brij-78, 6%Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.