Osteopontin/OPN 蛋白, Rat (CHO, His)

Osteopontin/OPN Protein, a key non-collagenous bone protein, strongly binds to hydroxyapatite, crucial for the mineralized matrix.It likely plays a vital role in mediating cell-extracellular matrix interactions.Additionally, as a cytokine, OPN stimulates interferon-gamma and interleukin-12 production, while inhibiting interleukin-10, pivotal for the type I immunity pathway.Osteopontin/OPN Protein, Rat (CHO, His) is the recombinant rat-derived Osteopontin/OPN protein, expressed by CHO , with C-6*His labeled tag.

骨桥蛋白 (OPN) 是一种重要的非胶原骨蛋白，与羟基磷灰石紧密结合，在矿化基质中发挥着至关重要的作用。它可能对于促进细胞和细胞外基质之间的相互作用至关重要。此外，OPN 作为细胞因子，促进干扰素-γ 和白细胞介素 12 的产生，同时抑制白细胞介素 10 的产生。这些功能在导致 I 型免疫的途径中至关重要。

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10^5 cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37°C. The ED₅₀ for this effect is 1.281 μg/ml, corresponding to a specific activity is 780.640 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10⁵ cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37°C. The ED₅₀ for this effect is 1.281 μg/ml, corresponding to a specific activity is 780.640 units/mg.

Rat

CHO

C-6*His

P08721 (L17-N317)

25353  [NCBI]

LPVKVAEFGSSEEKAHYSKHSDAVATWLKPDPSQKQNLLAPQNSVSSEETDDFKQETLPSNSNESHDHMDDDDDDDDDGDHAESEDSVNSDESDESHHSDESDESFTASTQADVLTPIAPTVDVPDGRGDSLAYGLRSKSRSFPVSDEQYPDATDEDLTSRMKSQESDEAIKVIPVAQRLSVPSDQDSNGKTSHESSQLDEPSVETHSLEQSKEYKQRASHESTEQSDAIDSAEKPDAIDSAERSDAIDSQASSKASLEHQSHEFHSHEDKLVLDPKSKEDDRYLKFRISHELESSSSEVN

approximately 34.83 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.