OX40 Ligand/TNFSF4 蛋白, Human (HEK293, Fc)

OX40 Ligand (TNFSF4) is a type II glycoprotein with a cytoplasmic tail of 23 aa and an extracellular domain of 133 aa^[1]. OX40 Ligand is a ligand for TNFRSF4 (CD134), belongs to tumor necrosis factor (TNF) family. OX40 Ligand can activate OX40 and thereby functioning as a T cell co-stimulatory molecule. The OX40-OX40 Ligand interaction promotes effector T-cell survival and effectively induces memory T-cell generation, as well as enhances the helper function of Tfh for B cells, and also promotes the differentiation and maturation of DCs^[1][2]. OX40 Ligand/TNFSF4 Protein, Human (HEK293, Fc) is a recombinant human OX40 Ligand (Q51-L183) with N-terminal hFc tag, which is produced in HEK293.

OX40 配体 (TNFSF4) 是一种 II 型糖蛋白，具有 23 个氨基酸的胞质尾部和 133 个氨基酸的胞外结构域^[1]。OX40 配体在抗原呈递细胞上表达，例如 B 细胞、树突状细胞 (DC) 和巨噬细胞以及气道平滑肌细胞^[3]。OX40 Ligand是TNFRSF4 (CD134)的配体，属于肿瘤坏死因子(TNF)家族。
OX40 Ligand可以激活OX40，从而起到T细胞共刺激分子的作用。OX40-OX40配体相互作用促进效应T细胞存活并有效诱导记忆T细胞生成，增强Tfh对B细胞的辅助功能，并促进DC的分化和成熟^{[1]< /sup>[2]}。
人OX40配体与小鼠、大鼠和兔的氨基酸序列一致性<70%。
OX40配体与OX40之间的相互作用对于抗原特异性记忆T细胞的生成至关重要，并诱导宿主抗肿瘤免疫^[4]。但 OX40 和 OX40L 的过度上调可能会导致 Tfh 细胞异常激活和自身抗体过度产生，从而导致自身免疫性疾病^[1]。

OX4 Ligand (human, 1 ng/mL, 7 days, added into DC-T cell cultures at days and 4) inhibits the generation of IL-1-producing T cells induced by immature DCs and DCs treated with IL-1 and IFN-α^[5].

Loaded Recombinant Human OX40 Ligand/TNFSF4 Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human OX40/TNFRSF4 Protein, His Tag with an affinity constant of 0.909 μM as determined in BLI assay.

Human

HEK293

N-hFc

P23510 (Q51-L183)

7292  [NCBI]

Approximately 46.6 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.